Pax4-cre transgenic mice have the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a Tet-off cassette (EGFP/tTA). The transgene also contains a tetracycline-responsive element with a CMV minimal promoter (tetO-CMVmin) driving expression of a Cre-recombinase. The Pax4 promoter drives expression of Cre in pancreatic progenitor cells. The donating investigator confirms the absence of GFP expression, tTA activity, and tetracycline-dependant inhibition of Cre recombinase in this transgenic strain. While not useful as a Tet-Off tool, these mice may be useful as for studying pancreatic islet development and beta cell differentiation.
Douglas A Melton, Harvard University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Pax4-cre transgenic mice have the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a Tet-off cassette (EGFP/tTA). The transgene also contains a tetracycline-responsive element with a CMV minimal promoter (tetO-CMVmin) driving expression of a Cre-recombinase gene. In the absence of tetracycline/doxycycline, Cre recombinase expression is observed in Pax4-expressing cells (pancreatic progenitor cells). While designed to concomitantly allow tetracycline/doxycycline-dependant inhibition of Cre recombinase expression, the donating investigator confirms no such inhibition is observed. Also, no GFP expression is observed with or without tetracycline/doxycycline administration. Therefore, Pax4-cre transgenic mice function only for Cre recombinase expression in Pax4-expressing cells. When bred with mice containing a loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Pax4-expressing cells of the offspring. While not useful as a Tet-Off tool, these mice may be useful as for studying pancreatic islet development and beta cell differentiation.
For example, when bred to STOCK Gt(ROSA)26Sortm1(Notch1)Dam/J mice (Stock No. 008159), Notch activation inhibits the differentiation of pancreatic progenitor cells into α and β endocrine cells and shunts them instead toward a duct fate.
The Pax4-cre transgene was designed with the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a Tet-off cassette (EGFP/tTA). The transgene also contained a tetracycline-responsive element with a CMV minimal promoter (tetO-CMVmin) driving expression of a Cre-recombinase. Of note, these Pax4-cre transgenic mice function only for Cre recombinase expression in Pax4-expressing cells (no Tet-Off or fluorescent function is reported: see strain phenotype description for more details). The transgene was microinjected into fertilized (C57BL/6 X CBA) F1 oocytes, and founder mice were bred to ICR outbred mice to establish a colony. Upon arrival at The Jackson Laboratory, transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression |
Allele Name | transgene insertion 1, Douglas A Melton |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Pax4-Cre |
Gene Symbol and Name | Tg(Pax4-cre)1Dam, transgene insertion 1, Douglas A Melton |
Gene Synonym(s) | |
Promoter | Pax4, paired box 4, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Strain of Origin | (C57BL/6 x CBA)F1 |
Chromosome | UN |
General Note | These Pax4-cre transgenic mice function only for cre recombinase expression in Pax4-expressing cells (no tet-Off or fluorescence function is reported). The transgene was microinjected into fertilized (C57BL/6 X CBA)F1 oocytes, and founder mice were bred to ICR outbred mice to establish a colony. |
Molecular Note | The Pax4-cre transgene was designed with the mouse paired box gene 4 (Pax4) promoter directing expression of an enhanced green fluorescent protein fused to a tet-off cassette (EGFP/tTA). The transgene also contained a tetracycline-responsive element witha CMV minimal promoter (tetO-CMVmin) driving expression of a cre-recombinase. |
Mutations Made By | Douglas Melton, Harvard University |
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony, or with C57BL/6J inbred mice.
When using the STOCK Tg(Pax4-cre)1Dam/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014158 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non carrier for Tg(Pax4-cre)1Dam |
Frozen Mouse Embryo | STOCK Tg(Pax4-cre)1Dam/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pax4-cre)1Dam/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Pax4-cre)1Dam/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Pax4-cre)1Dam/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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