B6;129-Nrxn3^tm3Sud/J

Stock number: 014157
Product name: N3F
Research areas: Neurobiology Research, Research Tools

Description

Exon 18 of these Nrxn3 (neurexin III) targeted mutant mice is flanked by loxP sites. This strain may be useful in generating tissue-specific alpha and beta isoform knock-outs for use in a variety of neurobiological studies.

Detailed description

These mice carry a floxed allele of the Nrxn3 (neurexin III) gene. When bred to mice that express Cre recombinase, resulting offspring will have exon 18 deleted in the cre-expressing tissues, blocking expression of the alpha and beta transcripts. Homozygous floxed mice are viable, fertile and do not display any gross physical or behavioral abnormalities. Gene expression of the floxed allele is reportedly normal in brain tissue.

Development

Exon 18 (the first common coding exon for the alpha and beta transcripts) was flanked by loxP sites and an FRT-flanked neomycin selection cassette was placed in the 5' intron. The targeting construct was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant mice were crossed with a C57BL/6-129 mixed background Flp transgenic mouse to excise the neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Allele

Symbol: Nrxn3^tm3Sud
Name: targeted mutation 3, Thomas C Sudhof
MGI accession ID: MGI:4941813
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Nrxn3
Marker name: neurexin III
Chromosome: 12
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 18 (the first common coding exon for the alpha and beta transcripts) was flanked by loxP sites and an FRT-flanked neomycin selection cassette was placed in the 5' intron. Flp-mediated recombination removed the neo cassette.