Exon 18 of these Nrxn3 (neurexin III) targeted mutant mice is flanked by loxP sites. This strain may be useful in generating tissue-specific alpha and beta isoform knock-outs for use in a variety of neurobiological studies.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
These mice carry a floxed allele of the Nrxn3 (neurexin III) gene.
When bred to mice that express Cre recombinase, resulting offspring will have exon 18 deleted in the cre-expressing tissues, blocking expression of the alpha and beta transcripts. Homozygous floxed mice are viable, fertile and do not display any gross physical or behavioral abnormalities. Gene expression of the floxed allele is reportedly normal in brain tissue.
Exon 18 (the first common coding exon for the alpha and beta transcripts) was flanked by loxP sites and an FRT-flanked neomycin selection cassette was placed in the 5' intron. The targeting construct was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were crossed with a C57BL/6-129 mixed background Flp transgenic mouse to excise the neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
|Allele Name||targeted mutation 3, Thomas C Sudhof|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Nrxn3, neurexin III|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exon 18 (the first common coding exon for the alpha and beta transcripts) was flanked by loxP sites and an FRT-flanked neomycin selection cassette was placed in the 5' intron. Flp-mediated recombination removed the neo cassette.|
|Mutations Made By|| |
Dr. Thomas Sudhof, Stanford University School of Medicine
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the N3F mouse strain in a publication, please cite the originating article(s) and include JAX stock #014157 in your Materials and Methods section.