These floxed Cdk5 (cyclin-dependent kinase 5) mice are useful for generating tissue-specific knockouts which may be useful in studies of synapse formation.
Li-Huei Tsai, Massachusetts Institue of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Cdk5 | cyclin-dependent kinase 5 |
These Cdk5 floxed mutant mice possess loxP sites flanking exons 1-5. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring lack detectable Cdk5 in the cre-expressing tissues.
Exons 1-5 of the targeted gene were flanked by loxP sites and a FRT-flanked neomycin cassette was placed immediately 3'. The targeting construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Resultant chimeric mice were crossed to C57BL/6 background Flp transgenic mice to remove the neomycin drug selection cassette. The strain was backcrossed to C57BL/6 (see SNP note below) for six or seven generations by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Allele Name | targeted mutation 1.1, Li-Huei Tsai |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Cdk5fl |
Gene Symbol and Name | Cdk5, cyclin-dependent kinase 5 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 5 |
Molecular Note | Exons 1-5 of the targeted gene were flanked by loxP sites and a FRT-flanked neomycin cassette was placed immediately 3'. Flp-mediated recombination removed the neo cassette. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S4(Cg)-Cdk5tm1.1Lht/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014156 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Cdk5<tm1.1Lht> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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