These floxed Cdk5 (cyclin-dependent kinase 5) mice are useful for generating tissue-specific knockouts which may be useful in studies of synapse formation.
Li-Huei Tsai, Massachusetts Institue of Technology
These Cdk5 floxed mutant mice possess loxP sites flanking exons 1-5. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express Cre recombinase, resulting offspring lack detectable Cdk5 in the cre-expressing tissues.
Exons 1-5 of the targeted gene were flanked by loxP sites and a FRT-flanked neomycin cassette was placed immediately 3'. The targeting construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Resultant chimeric mice were crossed to C57BL/6 background Flp transgenic mice to remove the neomycin drug selection cassette. The strain was backcrossed to C57BL/6 (see SNP note below) for six or seven generations by the donating laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Allele Name||targeted mutation 1.1, Li-Huei Tsai|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cdk5, cyclin-dependent kinase 5|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Exons 1-5 of the targeted gene were flanked by loxP sites and a FRT-flanked neomycin cassette was placed immediately 3'. Flp-mediated recombination removed the neo cassette.|
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129S4(Cg)-Cdk5tm1.1Lht/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014156 in your Materials and Methods section.