NOD.B6-Ptprc^b/6908MrkTacJ

Stock number: 014149
Product name: NOD.B6-Cd45.2 line 6908
Strain synonyms: NOD.Cd45.2
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This NOD congenic strain contains Ptprc of C57BL/6J origin (CD45.2) and is reported to exhibit a high frequency of diabetes. As Ptprc is expressed on all adaptive and innate immune cells, this strain provides a unique cell surface marker that can be used to track lineage and phenotype of all hematopoietic donor derived or transferred cells making it useful in studies monitoring immune pathogenesis in the NOD model.

 

Detailed description

This NOD congenic strain, commonly referred to as NOD.Cd45.2, is viable and fertile. Mice of this strain contain a genomic segment from the diabetes resistant strain C57BL/6 introgressed into the NOD chromosome 1. The C57BL/6 genomic segment includes Ptprc^b, commonly referred to as Cd45.2. Although reported to be significantly reduced compared to NOD, congenic female mice exhibit a high frequency of diabetes with about 60% of the females becoming diabetic by 200 days of age. As Ptprc is expressed on all adaptive and innate immune cells, this strain provides a unique cell surface marker that can be used to track lineage and phenotype of all hematopoietic donor derived or transferred cells making it useful in studies monitoring immune pathogenesis in the NOD model.

Development

This strain, commonly called NOD-CD45.2, was produced by mating mice from NOD/Jrc (Ptprc^a) to C57BL/6 (Ptprc^b) followed by 10 additional marker assisted crosses to NOD/Jrc with selection for Ptprc^b on chromosome 1. Five (5) additional crosses to NOD/MrkTac were completed to shorten the chromosome 1 congenic interval in the Ptprc^b region. Recent SNP testing (direct data submission) indicates that the congenic interval is less that 0.84Mb, with markers rs32090581 (139.65Mb) and rs32478398 (140.41Mb) to be of C57BL/6 origin; while markers D1Mit537 (139.62) and rs30655958 (140.49Mb) are of NOD origin. In 2010, the Type 1 Diabetes Resource received this homozygous strain at N16F?

Allele

Symbol: Ptprc^b
Name: b variant
MGI accession ID: MGI:4819850
Generation method: Not Applicable
Marker symbol: Ptprc
Marker name: protein tyrosine phosphatase, receptor type, C
Chromosome: 1
Strain of origin: Not Applicable
Site of expression: Widely expressed on all adaptive and innate immune cells.
Molecular note: Ptprc^b is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485).

Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).