This NOD congenic strain contains Ptprc of C57BL/6J origin (CD45.2) and is reported to exhibit a high frequency of diabetes. As Ptprc is expressed on all adaptive and innate immune cells, this strain provides a unique cell surface marker that can be used to track lineage and phenotype of all hematopoietic donor derived or transferred cells making it useful in studies monitoring immune pathogenesis in the NOD model.
Linda Wicker, University of Cambridge, UK
This NOD congenic strain, commonly referred to as NOD.Cd45.2, is viable and fertile. Mice of this strain contain a genomic segment from the diabetes resistant strain C57BL/6 introgressed into the NOD chromosome 1. The C57BL/6 genomic segment includes Ptprcb, commonly referred to as Cd45.2. Although reported to be significantly reduced compared to NOD, congenic female mice exhibit a high frequency of diabetes with about 60% of the females becoming diabetic by 200 days of age.
As Ptprc is expressed on all adaptive and innate immune cells, this strain provides a unique cell surface marker that can be used to track lineage and phenotype of all hematopoietic donor derived or transferred cells making it useful in studies monitoring immune pathogenesis in the NOD model.
This strain, commonly called NOD-CD45.2, was produced by mating mice from NOD/Jrc (Ptprca) to C57BL/6 (Ptprcb) followed by 10 additional marker assisted crosses to NOD/Jrc with selection for Ptprcb on chromosome 1. Five (5) additional crosses to NOD/MrkTac were completed to shorten the chromosome 1 congenic interval in the Ptprcb region. Recent SNP testing (direct data submission) indicates that the congenic interval is less that 0.84Mb, with markers rs32090581 (139.65Mb) and rs32478398 (140.41Mb) to be of C57BL/6 origin; while markers D1Mit537 (139.62) and rs30655958 (140.49Mb) are of NOD origin. In 2010, the Type 1 Diabetes Resource received this homozygous strain at N16F?
|Allele Name||b variant|
|Allele Type||Not Applicable|
|Allele Synonym(s)||CD45.2; Ly5b|
|Gene Symbol and Name||Ptprc, protein tyrosine phosphatase, receptor type, C|
|Site of Expression||Widely expressed on all adaptive and innate immune cells.|
|Strain of Origin||Not Applicable|
|Molecular Note||Ptprcb is found in strains C57BL/6, C3H/An, DBA/2, AKR, and many others (J:13367, J:12054, J:12077, J:8603). Twelve nucleotide differences between the a and b alleles have been identified. These base substitutions correspond to five amino-acid changes within the extracellular domain of the encoded protein. These amino-acid differences are clustered in a region that also contains the greatest divergence between mouse and rat sequences (J:22485). |
Note that the allele designations originally described were reversed in 1987 (J:8603); all publications prior to 1987 show SJL/J, STS/A, and DA as having the b allele and the C57BL/6J group as having the a allele (J:22341).
While maintaining a live colony, this strain is mated homozygote x homozygote.
A footpad injection of Complete Freund s Adjuvant (CFA) administered once at weaning will delay diabetes onset, thus extending the lifespan of breeders. Use of Complete Freund s Adjuvant in NOD mice can be found in Current Protocols in Immunology page 15.9.19, Reproduction.
When using the NOD.B6-Cd45.2 line 6908 mouse strain in a publication, please cite the originating article(s) and include JAX stock #014149 in your Materials and Methods section.