C57BL/6-Itgal^tm1.1Mshi/J

Stock number: 014148
Research areas: Cell Biology Research, Immunology, Inflammation and Autoimmunity Research

Description

This Itgal (integrin alpha L) I306A mutant strain may be useful in studies of integrin function.

Detailed description

This strain carries an I306A knock-in mutation of the Itgal (integrin alpha L; also known as Cd11a or LFA-1) gene. This mutation was shown to perturb lymphocyte de-adhesion that is part of circulating T cell homing/rolling to lymph nodes via endothelial venules. Homozygotes are viable and fertile and do not exhibit any gross abnormalities or developmental defects except for a smaller sized peripheral lymph nodes which contain fewer lymphocytes as compared to wildtype mice. Lymphocytes from these mice show reduced expression of ITGAL, but not other integrins. These mice may be useful in studies of integrin function.

Development

A targeting vector containing an I306A mutation in exon 9 and a self-excising loxP-flanked ACE (angiotensin-converting enzyme promoter)-cre-neomycin resistance cassette (also called ACN) in intron 9 was introduced to Bruce-4 C57BL/6-derived embryonic stem cells. Deletion of the floxed segment occurred during spermatogenesis, leaving a single loxP site. This strain was maintained on a C57BL/6 background by the donating laboratory.

Allele

Symbol: Itgal^tm1.1Mshi
Name: targeted mutation 1.1, Motomu Shimaoka
MGI accession ID: MGI:4888587
Generation method: Targeted
Attributes: Not Specified
Synonyms: Itgal^I306A; Itgal^{tm1(I306A)Mshi}
Marker symbol: Itgal
Marker name: integrin alpha L
Marker synonyms: CD11A; LFA-1; LFA1A; Ly-15; Ly-21
Chromosome: 7
Strain of origin: B6.Cg-Thy1^a
Molecular note: A targeting vector containing an I306A mutation in exon 9 and a self-excising loxP-flanked ACE (angiotensin-converting enzyme promoter)-cre-neomycin resistance cassette (also called ACN) in intron 9 was introduced to Bruce-4 C57BL/6-derived embryonic stem cells. Deletion of the floxed segment occurred during spermatogenesis, leaving a single loxP site.