This Itgal (integrin alpha L) I306A mutant strain may be useful in studies of integrin function.
Motomu Shimaoka, Immune Disease Institute (formerly CBRI)
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Itgal | integrin alpha L |
This strain carries an I306A knock-in mutation of the Itgal (integrin alpha L; also known as Cd11a or LFA-1) gene. This mutation was shown to perturb lymphocyte de-adhesion that is part of circulating T cell homing/rolling to lymph nodes via endothelial venules. Homozygotes are viable and fertile and do not exhibit any gross abnormalities or developmental defects except for a smaller sized peripheral lymph nodes which contain fewer lymphocytes as compared to wildtype mice. Lymphocytes from these mice show reduced expression of ITGAL, but not other integrins. These mice may be useful in studies of integrin function.
A targeting vector containing an I306A mutation in exon 9 and a self-excising loxP-flanked ACE (angiotensin-converting enzyme promoter)-cre-neomycin resistance cassette (also called ACN) in intron 9 was introduced to Bruce-4 C57BL/6-derived embryonic stem cells. Deletion of the floxed segment occurred during spermatogenesis, leaving a single loxP site. This strain was maintained on a C57BL/6 background by the donating laboratory.
Allele Name | targeted mutation 1.1, Motomu Shimaoka |
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Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | ItgalI306A; Itgaltm1(I306A)Mshi |
Gene Symbol and Name | Itgal, integrin alpha L |
Gene Synonym(s) | |
Promoter | Itgal, integrin alpha L, mouse, laboratory |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 7 |
Molecular Note | A targeting vector containing an I306A mutation in exon 9 and a self-excising loxP-flanked ACE (angiotensin-converting enzyme promoter)-cre-neomycin resistance cassette (also called ACN) in intron 9 was introduced to Bruce-4 C57BL/6-derived embryonic stem cells. Deletion of the floxed segment occurred during spermatogenesis, leaving a single loxP site. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the C57BL/6-Itgaltm1.1Mshi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014148 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Itgal<tm1.1Mshi> |
Frozen Mouse Embryo | C57BL/6-Itgal<tm1.1Mshi>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Itgal<tm1.1Mshi>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Itgal<tm1.1Mshi>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Itgal<tm1.1Mshi>/J | $3373.50 |
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