B6.Cg-Tg(Ckm-DYSF)3Kcam/J

Stock number: 014146

Description

Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. This strain may be useful for studies of dysferlin and muscular function.

Detailed description

Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of dysferlin in skeletal muscle is approximately 4-8 fold that of the endogenous protein level. There is no detectable muscle pathology up to one year of age.

Development

The human DYSF (dysferlin) cDNA with SV40 polyA was inserted downstream of the mouse Ckm (creatine kinase, muscle; MCK) promoter. The transgenic vector was introduced to B6SJLF1 hybrid embryos to create the mutation. This strain was backcrossed to C57BL/6 for six generations by the donating laboratory.

Allele

Symbol: Tg(Ckm-DYSF)3Kcam
Name: transgene insertion 3, Kevin P Campbell
MGI accession ID: MGI:4849285
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Synonyms: MCKhFerL1L
Marker symbol: Tg(Ckm-DYSF)3Kcam
Marker name: transgene insertion 3, Kevin P Campbell
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: DYSF,dysferlin,human
Molecular note: The human DYSF (dysferlin) cDNA with SV40 polyA was inserted downstream of the mouse Ckm (creatine kinase, muscle; MCK) promoter. Line 3 was generated.