Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. This strain may be useful for studies of dysferlin and muscular function.
Kevin Campbell, University of Iowa
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Expression of human DYSF (dysferlin) cDNA is driven by the mouse Ckm (creatine kinase, muscle; MCK) promoter in this transgenic strain. Hemizygotes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of dysferlin in skeletal muscle is approximately 4-8 fold that of the endogenous protein level. There is no detectable muscle pathology up to one year of age.
The human DYSF (dysferlin) cDNA with SV40 polyA was inserted downstream of the mouse Ckm (creatine kinase, muscle; MCK) promoter. The transgenic vector was introduced to B6SJLF1 hybrid embryos to create the mutation. This strain was backcrossed to C57BL/6 for six generations by the donating laboratory.
Expressed Gene | DYSF, dysferlin, human |
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Site of Expression |
Allele Name | transgene insertion 3, Kevin P Campbell |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | MCKhFerL1L |
Gene Symbol and Name | Tg(Ckm-DYSF)3Kcam, transgene insertion 3, Kevin P Campbell |
Gene Synonym(s) | |
Promoter | Ckm, creatine kinase, muscle, mouse, laboratory |
Expressed Gene | DYSF, dysferlin, human |
Strain of Origin | (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | The human DYSF (dysferlin) cDNA with SV40 polyA was inserted downstream of the mouse Ckm (creatine kinase, muscle; MCK) promoter. Line 3 was generated. |
Mutations Made By | Kevin Campbell, University of Iowa |
When maintained as a live colony, hemizygotes may be bred.
When using the B6.Cg-Tg(Ckm-DYSF)3Kcam/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014146 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ckm-DYSF)3Kcam |
Frozen Mouse Embryo | B6.Cg-Tg(Ckm-DYSF)3Kcam/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ckm-DYSF)3Kcam/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Ckm-DYSF)3Kcam/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Ckm-DYSF)3Kcam/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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