Mice homozygous for the ENU-induced Adam17 (wavedx) mutation are characterized by open eyelids and curly vibrissae at birth; adult animals display wavy coats, corneal opacities, are susceptible to dextran sulfate sodium (DSS)-induced colitis. This mutant mouse strain may be useful in studies of inflammatory bowel disease.
Bruce Beutler, University of Texas Southwestern Medical
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Chemically induced (ENU) | Adam17 | a disintegrin and metallopeptidase domain 17 |
Mice homozygous for the mutation are viable and are characterized by open eyelids and curly vibrissae at birth; adult animals display wavy coats and corneal opacities. These animals are susceptible to dextran sulfate sodium (DSS)-induced colitis and exhibit severe weight loss as a result of DSS administration. The donating investigator indicates that homozygous males are fertile, however homozygous females do not breed. This mutant mouse strain may be useful in studies of inflammatory bowel disease.
For additional information, see The Scripps Research Institute Mutagenix website.
Heterozygotes: Not evaluated, phenotype is presumed to be normal.
This missense point mutation was generated by ethylnitrosourea (ENU) mutagenesis in C57BL/6J males. Mutagenized males were outcrossed to C57BL/6J females. The mutation results in a T to A transversion at position 1186 of the
Adam17 gene, in exon 9 of 19 total exons. The mutation alters the corresponding amino acid from phenylalanine to isoleucine at codon 343. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | mutation 1, Bruce Beutler |
---|---|
Allele Type | Chemically induced (ENU) |
Allele Synonym(s) | wavedX |
Gene Symbol and Name | Adam17, a disintegrin and metallopeptidase domain 17 |
Gene Synonym(s) | |
Strain of Origin | C57BL/6J |
Chromosome | 12 |
Molecular Note | The wavedX mutation has been identified as a T to A transversion at nucleotide position 1186 of the gene, in the ninth of its 19 exons. It changes phenylalanine to isoleucine at amino acid position 343 of the protein (F343I). |
Mutations Made By | Bruce Beutler, University of Texas Southwestern Medical |
While maintaining a live colony, these mice are bred as heterozygote female x homozygote male. Females homozygous for the mutation do not breed.
When using the wavedx mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #34295 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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