These mutant mice possess loxP sites flanking exons 11-12 of the Rbfox1 gene. This strain may be useful in generating conditional mutations for studying the role of Fox-1 in development and physiology of the brain, heart, and muscle tissues.
Douglas Black, UCLA
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Rbfox1 | RNA binding protein, fox-1 homolog (C. elegans) 1 |
These Fox1flox mutant mice possess loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. Fox-1 is expressed in brain, heart, and skeletal muscle and regulates alternative splicing in vertebrates by binding specifically to (U)GCAUG sequences. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 11-12 deleted in the Cre-expressing tissue, resulting in inactivation of Fox-1 gene function.
For example, when crossed to a strain expressing Cre recombinase in the central and peripheral nervous system (see Stock No. 003771), this mutant mouse strain may be useful in studies of neuronal excitation and seizures.
When bred to mice carrying Tg(Pcp2-cre)2Mpin (Stock No. 004146) and Rbfox2tm1.1Dblk (Stock No. 014090) , Cre recombinase expression in the Purkinje cells may be useful in studies of Purkinje cell pacemaking.
A targeting vector was designed to insert loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene, followed by a frt-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S2/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and resulting chimeric males were bred to C57BL/6J females. Offspring were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J transgenic mice (Stock No. 003946) to delete the neo cassette, resulting in a colony of Fox-1flox mice. The donating investigator reported that the resulting offspring were then backcrossed to C57BL/6J mice for at least 6 generations (see SNP note below). Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1.1, Douglas Black |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Fox-1flox; Rbfox1loxP |
Gene Symbol and Name | Rbfox1, RNA binding protein, fox-1 homolog (C. elegans) 1 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 16 |
Molecular Note | A targeting vector was designed to insert loxP sites flanking exons 11-12 of the RNA binding protein, fox-1 homolog 1 (Rbfox1) gene, followed by a frt-flanked neomycin resistance (neo) cassette. Flp-mediated recombination removed the neo cassette leaving exons 11 and 12 floxed. |
Mutations Made By | Douglas Black, UCLA |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6.129S2-Rbfox1tm1.1Dblk/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014089 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Rbfox1<tm1.1Dblk> |
Frozen Mouse Embryo | B6.129S2-Rbfox1<tm1.1Dblk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Rbfox1<tm1.1Dblk>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S2-Rbfox1<tm1.1Dblk>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S2-Rbfox1<tm1.1Dblk>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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