Expression of Cacna1g is regulated by a tetracycline operator (tetO) in this transgenic strain which may be useful in studies of cardiomyopathy.
Jeffery D. Molkentin, Cincinnati Children's Hospital
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inducible, Inserted expressed sequence) |
Expression of Cacna1g (calcium channel, voltage-dependent, T type, alpha 1G subunit) is regulated by a tetracycline operator (tetO); also called tetracycline-responsive element (TRE, TetRE) or tet-operator) in this transgenic strain. When mated to a mutant strain expressing tetracycline-controlled transactivator protein (tTA), expression of CACNA1G protein may be regulated with tetracycline or its analog doxycycline (dox) in the double mutant offspring. When crossed with a Myh6-tTA strain (e.g. Stock No. 003170), double transgenic mice show protection from cardiac hypertrophy after transverse aortic constriction (TAC) surgery. This strain may be useful in studies of cardiomyopathy.
A transgenic vector carrying mouse Cacna1g cDNA under the control of a tetO promoter was microinjected into FVB fertilized oocytes. The strain was maintained on an FVB background by the donating laboratory.
Expressed Gene | Cacna1g, calcium channel, voltage-dependent, T type, alpha 1G subunit, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 1, Jeffery D Molkentin |
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Allele Type | Transgenic (Inducible, Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(tetO-Cacna1g)1Jmol, transgene insertion 1, Jeffery D Molkentin |
Gene Synonym(s) | |
Promoter | tetO, tet operator, |
Expressed Gene | Cacna1g, calcium channel, voltage-dependent, T type, alpha 1G subunit, mouse, laboratory |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | The transgenic vector carried mouse Cacna1g cDNA under the control of a tetO promoter. Line 1 was generated. |
Mutations Made By | Jeffery Molkentin, Cincinnati Children's Hospital |
When maintained as a live colony, hemizygotes may be bred.
When using the FVB-Tg(tetO-Cacna1g)1Jmol/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013777 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(tetO-Cacna1g)1Jmol |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacna1g)1Jmol/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacna1g)1Jmol/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacna1g)1Jmol/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(tetO-Cacna1g)1Jmol/J | $3373.50 |
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