STOCK Hprt1^{tm57(Ple26-lacZ)Ems}/Mmjax

Stock number: 013764
Product name: CCL27-lacZ
Research areas: Neurobiology Research, Research Tools

Description

These mice have the Ple26-lacZ transgene targeted as a single copy "knockin" into the upstream region of the Hprt1 locus on the X chromosome. The promoter/regulatory regions of the human CCL27 gene (chemokine (C-C motif) ligand 27) direct expression of β-galactosidase.

Allele

Symbol: Hprt1^{tm57(Ple26-lacZ)Ems}
Name: targeted mutation 57, Elizabeth M Simpson
MGI accession ID: MGI:4836990
Generation method: Targeted
Marker symbol: Hprt1
Marker name: hypoxanthine phosphoribosyltransferase 1
Chromosome: X
Strain of origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed in throughout the brain.
Molecular note: The Ple26-lacZ transgene (pEMS1497) was designed with the 3252 bp Ple26 minipromoter (CCL27-A; derived from one upstream and two downstream putative regulatory subregions along with a section of the promoter from the human chemokine (C-C motif) ligand 27 [CCL27] gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.
General note: Germ line transmission of mutant cell line mEMS4649 has been established.