Overview

This strain carries multiple undefined mutations identified in a screen of ENU-induced mutations and may be useful in studies of congenital heart disease and cystic kidney disease.

The Jackson Laboratory cannot guarantee that cryorecovery of G1 sperm from the Bench to Bassinet (B2B) collection will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser.

Donating Investigator

Cecilia Lo, Univ of Pittsburgh School of Medicine

Genetic overview

Genetic Background	Generation

b2b243Clo

Allele Type	Gene Symbol	Gene Name
Chemically induced (ENU)	b2b243Clo	Mutant line 243

b2b243.1Clo

Allele Type	Gene Symbol	Gene Name
Chemically induced (ENU)	b2b243.1Clo	Mutant line 243.1

b2b243.2Clo

Allele Type	Gene Symbol	Gene Name
Chemically induced (ENU)	b2b243.2Clo	Mutant line 243.2

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Research Applications

Internal/Organ Research
Cardiovascular Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This strain, carrying undefined mutations b2b243.1Clo and b2b243.2Clo, was identified in an ENU screen for recessive cardiovascular development phenotypes in Dr. Cecilia Lo's laboratory, NHLBI Cardiovascular Development Consortium (CvDC). It was recovered from G1 sperm and associated with the phenotype described here. Because G1 sperm were cryopreserved, additional incidental mutations are also segregating in this strain.

Homozygotes of mutant type 1 demonstrate a truncus arteriosus (PTA) with right aortic arch. Mutant type 2 shows cystic kidney and hydronephrosis.

Development

This strain contains at least two undefined mutations (b2b243.1Clo and b2b243.2Clo) that were identified in an ENU screen for recessive cardiovascular development phenotypes. It was created and maintained on a C57BL/6J genetic background by the NHLBI Cardiovascular Development Consortium (CvDC), Bench to Bassinet Program.

Genetics

b2b243Clo

Allele Symbol: b2b243Clo

Allele Name

Bench to Bassinet Program (B2B/CVDC), mutation 243 Cecilia Lo

Allele Type

Chemically induced (ENU)

Allele Synonym(s)

Jupiter

Gene Symbol and Name

b2b243Clo, Mutant line 243

Gene Synonym(s)

Strain of Origin

C57BL/6J

Chromosome

UN

General Note

Summative Diagnosis:
Mutant Type 1: Cardiac phenotype: Persistant truncus arteriosus (PTA) with right aortic arch
Mutant Type 2: Non-cardiac phenotype: Cystic kidney and hydronephrosis

Fyler Codes
The Fyler code developed by The Boston Children's Heart Foundation in Boston Children's Hospital provides a hierarchical clinical diagnosis of congenital cardiovascular defects and other disorders. These codes are used to delineate pathology in the mutant mouse models that parallel human disease and can be cross referenced to the International Pediatric and Congenital Cardiac Code (IPCCC) (http://www.ipccc.net/).

Fyler Code ID	Code Description
2720	Right aortic arch
0500	Truncus arteriosus
4502	Hydronephrosis
4508	Polycystic kidney disease

Molecular Note

This ENU-induced mutation line was isolated in a screen at the University of Pittsburgh. More than one mutation in this line results in a discernible phenotype in a homozygous recessive screen. At least two segregating phenotype groups are identified. See b2b243.1Clo and b2b243.1Clo.

b2b243.1Clo

Allele Symbol: b2b243.1Clo

Allele Name

Bench to Bassinet Program (B2B/CVDC), mutation 243.1 Cecilia Lo

Allele Type

Chemically induced (ENU)

Allele Synonym(s)

Gene Symbol and Name

b2b243.1Clo, Mutant line 243.1

Gene Synonym(s)

Strain of Origin

C57BL/6J

Chromosome

UN

General Note

Summative Diagnosis:
Cardiac phenotype: Persistant truncus arteriosus (PTA) with right aortic arch

Fyler Codes
The Fyler code developed by The Boston Children's Heart Foundation in Boston Children's Hospital provides a hierarchical clinical diagnosis of congenital cardiovascular defects and other disorders. These codes are used to delineate pathology in the mutant mouse models that parallel human disease and can be cross referenced to the International Pediatric and Congenital Cardiac Code (IPCCC) (http://www.ipccc.net/).

Fyler Code ID	Code Description
2720	Right aortic arch
0500	Truncus arteriosus

Molecular Note

This ENU-induced mutation was isolated in a screen at the University of Pittsburgh. It is a subline of b2b243Clo.

b2b243.2Clo

Allele Symbol: b2b243.2Clo

Allele Name

Bench to Bassinet Program (B2B/CVDC), mutation 243.2 Cecilia Lo

Allele Type

Chemically induced (ENU)

Allele Synonym(s)

Gene Symbol and Name

b2b243.2Clo, Mutant line 243.2

Gene Synonym(s)

Strain of Origin

C57BL/6J

Chromosome

UN

General Note

Summative Diagnosis:
Non-cardiac phenotype: Cystic kidney and hydronephrosis

Fyler Codes
The Fyler code developed by The Boston Children's Heart Foundation in Boston Children's Hospital provides a hierarchical clinical diagnosis of congenital cardiovascular defects and other disorders. These codes are used to delineate pathology in the mutant mouse models that parallel human disease and can be cross referenced to the International Pediatric and Congenital Cardiac Code (IPCCC) (http://www.ipccc.net/).

Fyler Code ID	Code Description
4502	Hydronephrosis
4508	Polycystic kidney disease

Molecular Note

This ENU-induced mutation was isolated in a screen at the University of Pittsburgh. It is a subline of b2b243Clo.

Disease/Phenotype

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Internal/Organ Research
- Kidney Defects
  - polycystic kidney disease
Cardiovascular Research
- Vascular Defects
- Heart Abnormalities

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: b2b243Clo/b2b243Clo
C57BL/6J-b2b243Clo

cardiovascular system phenotype

persistent truncus arteriosis

right aortic arch

growth/size/body region phenotype

polycystic kidney

renal/urinary system phenotype

hydronephrosis

polycystic kidney

References

Additional - b2b243.1Clo related

Additional - b2b243.2Clo related

Additional - b2b243Clo related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

Heterozygotes are both viable and fertile.
- Additional Breeding and Husbandry Support
Citation
When using the C57BL/6-b2b243Clo/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013609 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
> >	The Jackson Laboratory cannot guarantee that cryorecovery of G1 sperm from the Bench to Bassinet (B2B) collection will be successful or that the anticipated phenotype or genotype will be obtained. The cryorecovery fee for this effort will not be refunded or prorated if the recovery is unsuccessful or is in any way unsatisfactory. Genotyping will be the responsibility of the Purchaser

Related Products and Services

Frozen Mouse Embryo	C57BL/6-b2b243Clo/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	C57BL/6-b2b243Clo/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	C57BL/6-b2b243Clo/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	C57BL/6-b2b243Clo/J Frozen Embryo	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

b2b243Clo

Allele Symbol: b2b243Clo

b2b243.1Clo

Allele Symbol: b2b243.1Clo

b2b243.2Clo

Allele Symbol: b2b243.2Clo

Disease Terms

Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: b2b243Clo/b2b243CloC57BL/6J-b2b243Clo

cardiovascular system phenotype

growth/size/body region phenotype

renal/urinary system phenotype

References

Additional - b2b243.1Clo related

Additional - b2b243.2Clo related

Additional - b2b243Clo related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Genotype: b2b243Clo/b2b243Clo
C57BL/6J-b2b243Clo