STOCK Dnm3^tm1.2Pdc/J

Stock number: 013543
Research areas: Cell Biology Research, Neurobiology Research

Description

Dnm3 (dynamin 3) is a neuron-specific protein involved with syanaptic vescicle endocytosis. These mice carry a Dnm3 (dynamin 3) knockout allele that worsens the Dnm1 (dynamin 1) knockout phenotype.

Detailed description

These mice carry a targeted mutation of the Dnm3 (dynamin 3) gene shown to block protein expression in brain, lung and testis (sites of major expression). Homozygotes are viable, fertile, and do not display any gross physical or behavioral abnormalities. Lack of this protein alone in mice does not produce an obvious pathological phenotype, but in combination with a Dnm1 (dynamin 1) knockout, it worsens the Dnm1 mutant phenotype. Double knockout mice die shortly after birth and their neurons show a more severe defect in compensatory synaptic vesicle endocytosis.

Development

Exon 2 of the targeted gene was flanked by a loxP site in intron 1 and a FRT-flanked PGK-Neo sequence followed by a loxP site in intron 2. The targeting vector was electroporated into C57BL/6J-129S1/Sv-p⁺Tyr⁺Kitl ⁺ hybrid embryonic stem cells. Male chimeric offspring were mated to a C57BL/6 background FLPe recombinase-expressing deleter strain to excise the PGK-Neo sequence, leaving exon 2 flanked by loxP sites. Mating to an ACTB-Cre deleter strain yielded the knockout allele. This strain was maintained on a mixed C57BL/6 and 129S1 genetic background by the donating laboratory.

Allele

Symbol: Dnm3^tm1.2Pdc
Name: targeted mutation 1.2, Pietro De Camilli
MGI accession ID: MGI:5285526
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dnm3
Marker name: dynamin 3
Chromosome: 1
Strain of origin: 129S1/Sv and C57BL/6
Molecular note: Cre-mediated recombination removed exon 2 and the neo cassette. The absence of protein expression was confirmed by western blot analysis on brain, testis, and lung extracts.