NOD.FVB-Tg(EIIa-cre)C5379Lmgd/J

Stock number: 013251
Product name: NOD.E2a-cre
Strain synonyms: E2a-Cre
Research areas: Research Tools

Description

These EIIa-Cre transgenic mice express Cre recombinase under the control of the adenovirus EIIa promoter. When crossed with a strain containing loxP site-flanked sequences, Cre-mediated recombination occurs in a wide range of tissues, including germ cells that transmit the genetic alteration to progeny.

Detailed description

This line carries a Cre transgene under the control of the adenovirus EIIa promoter that targets expression of Cre recombinase to the early mouse embryo. Cre expression is thought to occur prior to implantation in the uterine wall. A mosaic pattern of expression is commonly observed. Cre-mediated recombination occurs in a wide range of tissues, including the germ cells that transmit the genetic alteration to progeny.

View cre expression characterization on the FVB background.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The EIIa-Cre plasmid was constructed by replacing the human cytomegalovirus promoter in pBS185 with the adenovirus EIIa promoter from pEII-lacZ. cre activity present in zygotes of the EIIa-cre genotype was measured as follows. EIIa-cre/EIIa-cre homozygous animals were mated with +/alphaA-loxP-STOP-loxP-TAg heterozygous animals and the presence of lens tumors in double transgenic progeny, indicative of cre-mediated excision of STOP sequences and a concomitant activation of SV40 TAg, was scored. The allele originated on an FVB/N (Stock No. 003314) background prior to transfer to the NOD/ShiLtJ utilizing marker assisted technology. At generation N4, all markers outside of the Chr. 6 congenic region (SNPS 06-027868538-G, 06-042391222-G and 06-053482462-M) are of NOD origin.

Allele

Symbol: Tg(EIIa-cre)C5379Lmgd
Name: transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal
MGI accession ID: MGI:2137691
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(EIIa-cre)C5379Lmgd
Marker name: transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: widespread expression (including germline); mosaic pattern commonly observed
Molecular note: The adenovirus EIIa promoter directs expression of Cre recombinase in preimplantation mouse embryos. This transgene expresses Cre recombinase in nearly all tissues.