These H3f3bFL mutant mice possess loxP sites flanking exons 2-4 of the H3 histone, family 3b (H3f3b) gene. The basic nuclear protein, H3F3B, is one of the four core histones responsible for the nucleosome structure. This mutant mouse strain may be useful in studies of chromosomal structure and function.
Paul Knoepfler, UC Davis Genome Center
These H3f3bFL mutant mice possess loxP sites flanking exons 2-4 of the H3 histone, family 3b (H3f3b) gene. The basic nuclear protein, H3F3B, is one of the four core histones responsible for the nucleosome structure. Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in studies of chromosomal structure and function.
For example, when bred to mice carrying Tg(Zp3-cre)3Mrt, Cre recombinase expression in the oocyte results in reduced number of homozygotes, infertility, defective cell division and chromosome segregation.
The targeting vector contains a FRT-flanked neomycin resistance cassette 3' of exon 1 followed by loxP sites flanking exons 2-4. The construct was electroporated into C57BL/6NTac-derived JM8.F6 embryonic stem (ES) cells. Correctly targeted ES cells were used to generate chimeric mice and the donating investigator reported that these mice were crossed to C57BL/6N mice (see SNP note below). Heterozygotes were intercrossed to generate a homozygous colony. Upon arrival, mice were bred to C57BL/6N for at least 1 generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 1, Paul Knoepfler|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||H3f3b, H3 histone, family 3B|
|Strain of Origin||C57BL/6N|
|Molecular Note||The targeting vector contains a FRT-flanked neomycin resistance cassette 3' of exon 1 followed by loxP sites flanking exons 2-4.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the H3f3bFL mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32055 in your Materials and Methods section.