NOD.B6-Idd10^C57BL/6J Idd18.2^C57BL/6J Idd18.3^C57BL/6J/2412MrkTacJ

Stock number: 013187
Product name: NOD.B6-Idd10/18.2/18.3, R2412
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

This strain is one of a set of 3 NOD.B6 congenic Idd10 and Idd18 strains (Stock No. 013185, 013186). Stocks 13185, 13186 and 13187 resulted from crossing segments of C57BL/6J derived chromosome 3 into the NOD/MrkTac background. Mice of this strain contain C57BL/6J derived Idd10, Idd18.2 and Idd18.3 regions. These strains may be useful to better understand the variation of diabetic resistance conferred by Idd loci and may be useful for identifying diabetes susceptible candidate genes within the Idd18 subloci.

Detailed description

This NOD/MrkTac congenic strain, commonly referred to as NOD.B6-Idd10/18R53, line 2412 is viable and fertile. . Mice of this strain contain a genomic segment from the diabetes resistant strain C57BL/6J (Stock No. 000664) introgressed into the NOD chromosome 3. The C57BL/6 genomic segment includes Idd10, Idd18.2 and Idd18.3; while the Idd3, Idd17 and Idd18.1 regions are of NOD origin. The Idd10 region contains major candidate gene Cd101 (100Mb); and the recently identified 966Kb (108.3Mb-109.3Mb) Idd18.3 region contains 22 genes and 1 pseudogene. Thymocytes of 3-week old male congenic mice express 1.46 fold more full length VAV3 mRNA than thymocytes of line 7754 (Stock No. 013186); which carries a C57BL/6J derived Idd18.1 (Vav3), Idd18.2 and Idd18.3. Diabetes incidence overlaps with line 7754 and is reported to be about 54%.
This strain is useful for conducting mapping experiments to further define the Idd18.3 region.

Development

This strain was produced by mating mice from NOD/MrkTac (NOD) to C57BL/6J followed by 13 additional crosses to NOD with selection for chromosome 3, Idd10, Idd18.2 and Idd18.3 markers. Siblings carrying the C57BL/6J derived segments were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 36, identifies no C57BL/6J genetic contamination of other chromosomes. In 2010, the Type 1 Diabetes Resource received this strain at generation N14F?.

Allele

Symbol: Idd18.2^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:5288384
Generation method: QTL
Marker symbol: Idd18.2
Marker name: insulin dependent diabetes susceptibility 18.2
Chromosome: 3
Strain of origin: C57BL/6J
Molecular note: No published characterization of this QTL has yet appeared.
General note: Located between Idd10 and Idd18.3 on Chromosome 3.

Allele

Symbol: Idd18.3^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:5288387
Generation method: QTL
Marker symbol: Idd18.3
Marker name: insulin dependent diabetes susceptibility 18.3
Chromosome: 3
Strain of origin: C57BL/6J
Molecular note: Twenty-two genes are located in this QTL region.
General note: Located in a 996 Kb interval in the region between but not including AC093365_1 and AL845310_10. Twenty-two genes and one pseudogene are located in this region.

Allele

Symbol: Idd10^C57BL/6J
Name: C57BL/6J
MGI accession ID: MGI:3038835
Generation method: QTL
Marker symbol: Idd10
Marker name: insulin dependent diabetes susceptibility 10
Chromosome: 3
Strain of origin: C57BL/6J
Molecular note: This allele confers resistance to insulin dependent diabetes compared to NOD.
General note: Idd10 and Idd18 interact to promote disease resistance. Homozygosity for C57BL/6-derived alleles at both Idd10 and Idd18 confers significantly increased resistance to type 1 diabetes.

Idd10 and Idd17 interact to promote disease resistance. Homozygosity for C57BL/6-derived alleles at both Idd10 and Idd17 confers significantly increased resistance to type 1 diabetes.

Idd10 and Idd3 interact to promote disease resistance. Homozygosity for C57BL/6-derived alleles at both Idd10 and Idd3 confers significantly increased resistance to type 1 diabetes.