129S4/SvJaeSor-Mycl^tm1.1Rne/J

Stock number: 013184
Research areas: Cancer Research, Research Tools

Description

These L-Myc^flox mutant mice may be useful, in combination with other Myc knock-out models, for studying early embryonic growth and organogenesis of the brain, olfactory bulb, and lungs.

Detailed description

These L-Myc^flox mutant mice possess loxP sites flanking all three exons v-myc myelocytomatosis viral oncogene homolog 1 (Mycl1) targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have all three Mycl1 exons deleted in the cre-expressing tissue. The donating investigator states that this strain may be useful, in combination with other Myc knock-out models, for studying early embryonic growth and organogenesis of the brain, olfactory bulb, and lungs.

Development

A targeting vector was designed to insert a loxP site upstream of the start codon, and a second loxP site just downstream of exon 3 of the v-myc myelocytomatosis viral oncogene homolog 1 (Mycl1) targeted gene. A frt-flanked neomycin (neo) resistance cassette was located upstream of the distal loxP site. The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to 129S4 inbred mice. Offspring, heterozygous for this Mycl1 allele, were bred with Flp transgenic mice on a 129S4 background to delete the neo cassette. Progeny were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the floxed-Mycl1 (L-Myc^flox) allele. Upon arrival, mice were bred to 129S4/SvJaeJ inbred mice (Stock No. 009104) for at least one generation to establish the colony.

Allele

Symbol: Mycl^tm1.1Rne
Name: targeted mutation 1.1, Robert N Eisenman
MGI accession ID: MGI:4818995
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Mycl
Marker name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived
Chromosome: 4
Strain of origin: 129S4/SvJaeSor
Molecular note: A targeting vector was designed to insert a loxP site upstream of the start codon, and a second loxP site just downstream of exon 3 of the v-myc myelocytomatosis viral oncogene homolog 1 (Mycl1) targeted gene. A FRT-flanked neomycin (neo) resistance cassette was located upstream of the second loxP site. Flp mediated recombination removed the neo cassette.