STOCK Dclk2^tm1.2Jgg/J

Stock number: 013173
Research areas: Neurobiology Research

Description

Homozyous Dclk2 (doublecortin-like kinase 2) targeted mutation mice lack expression of the gene and show no significant phenotype in brain structure. This strain may be useful in studies of epilepsy.

Detailed description

Homozyous Dclk2 (doublecortin-like kinase 2) targeted mutation mice lack expression of the gene and show no significant phenotype in brain structure. This strain may be useful in studies of epilepsy.

When combined with a Dcx (doublecortin) knockout allele, compound mutant mice exhibit spontaneous seizures and morphological defects of hippocampal CA pyramidal cells. Most double mutants reportedly die at a young age (approximately 3 weeks).

Allele

Symbol: Dclk2^tm1.2Jgg
Name: targeted mutation 1.2, Joseph G Gleeson
MGI accession ID: MGI:3844726
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Dclk2
Marker name: doublecortin-like kinase 2
Chromosome: 3
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was inserted upstream of exon 2 and a floxed neomycin selection cassette was inserted downstream of the exon. Exon 2 was removed by crossing founder mice with transgenic mice that express cre recombinase in the germline removed exon 2, and led to an out-of-frame mutation and the appearance of a stop codon within exon 3. Immunoblot analysis indicated a reduction in protein expression in heterozygote brain, and absence of protein in homozygote brain.