These mice possess loxP sites on either side of exon 2 of the Dclk2 (doublecortin-like kinase 2) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of neurodevelopment and epilepsy.
Joseph G Gleeson, University of California, San Diego
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Dclk2 | doublecortin-like kinase 2 |
These mice possess loxP sites on either side of exon 2 of the Dclk2 (doublecortin-like kinase 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of neurodevelopment and epilepsy.
A loxP site was introduced to intron 1 and a loxP-flanked PGK-neomycin resistance cassette was placed in intron 2. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was maintained on a mixed genetic background composed of C57BL/6, 129, and Black Swiss by the donating laboratory.
Allele Name | targeted mutation 1, Joseph G Gleeson |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Dclk2, doublecortin-like kinase 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 3 |
Molecular Note | A loxP site was introduced to intron 1 and a loxP-flanked PGK-neomycin resistance cassette was placed in intron 2. |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Dclk2tm1Jgg/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013172 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wildtype for Dclk2<tm1Jgg> |
Frozen Mouse Embryo | STOCK Dclk2<tm1Jgg>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dclk2<tm1Jgg>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dclk2<tm1Jgg>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Dclk2<tm1Jgg>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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