STOCK Dclk2^tm1Jgg/J

Stock number: 013172
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of exon 2 of the Dclk2 (doublecortin-like kinase 2) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of neurodevelopment and epilepsy.

Detailed description

These mice possess loxP sites on either side of exon 2 of the Dclk2 (doublecortin-like kinase 2) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of neurodevelopment and epilepsy.

Development

A loxP site was introduced to intron 1 and a loxP-flanked PGK-neomycin resistance cassette was placed in intron 2. The mutation was created in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was maintained on a mixed genetic background composed of C57BL/6, 129, and Black Swiss by the donating laboratory.

Allele

Symbol: Dclk2^tm1Jgg
Name: targeted mutation 1, Joseph G Gleeson
MGI accession ID: MGI:4819277
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dclk2
Marker name: doublecortin-like kinase 2
Chromosome: 3
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP site was introduced to intron 1 and a loxP-flanked PGK-neomycin resistance cassette was placed in intron 2.