These transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse <i>Thy1</i>, thymus cell antigen 1, theta, promoter (Thy1.2).  Transgene expression in founder line CLM13 is detected at high levels in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum.  No expression is detected in the external plexiform layer, mitral cells or internal plexiform layer of the olfactory bulb, layer 1 of the neocortex, cerebellar granule or purkinje cells, or retinal biopolar and amacrine cells.  In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP.  As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases.  Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-].  Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.   The Donating Investigator reports that homozygotes have a lower reproduction rate than hemizygotes.

This thy1::Clomeleon, CLM13, mutant mouse strain is an effective tool for measuring changes of intracellular chloride ion concentration in neurons by fluorescence spectrophotometry, and may be useful in studies requiring synaptic inhibition imaging.

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