| Allele Name | transgene insertion 13, George Augustine |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | Tg(Thy1-CFP,Topaz)CLM13Gjau; Tg(Thy1-CFP,YFP)CLM13Gjau |
| Gene Symbol and Name | Tg(Thy1-Clomeleon)13Gjau, transgene insertion 13, George Augustine |
| Gene Synonym(s) | |
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory |
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| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish |
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| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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| Expressed Gene | Clomeleon, Clomeleon, |
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| Site of Expression | CFP and YFP are expression is dependent on chloride ion concentration in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum. |
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| Strain of Origin | ICR |
| Chromosome | UN |
| Molecular Note | A transgenic construct containing Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2) was injected into fertilized ICR mouse eggs. Founder line CLM13 was subsequently established. Transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM13 is detected at high levels in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum. No expression is detected in the external plexi form layer, mitral cells or internal plexiform layer of the olfactory bulb, layer 1 of the neocortex, cerebellar granule or purkinje cells, or retinal biopolar and amacrine cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. |
