B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J

Stock number: 013134
Research areas: Research Tools

Description

This transgenic 2.3ColGFP mutant mouse strain expresses Green Fluorescent Protein in osteoblasts and osteocytes and may be useful in studies of bone development and osteoblast lineages.

Detailed description

These transgenic mice express Enhanced Green Fluorescent Protein gene under the control of the 2.3 kb (from the upstream promoter sequence to 22300 bp) rat procollagen, type 1, alpha 1( Col1a1) promoter. In cultures of bone marrow stromal cells (MSC) isolated from mice 6-8 weeks of age, transgene expression is detected at day 14, before osteocalcin expression is detected, by Northern blot analysis. Fluorescence is detected in developing mineralized bone nodules in cultures of calvarial osteoblast cells isolated from neonates, and in osteocytes throughout adult cortical bone. No fluorescence is detected in dermal fibroblasts, bladder smooth muscle cells, peribronchial connective tissues, or periosteal fibroblasts from bone. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development

A transgenic construct containing an Enhanced Green Fluorescent Protein gene under the control of the 2.3 kb rat procollagen, type 1, alpha 1( Col1a1) promoter (from the upstream promoter sequence to 22300 bp) was injected into fertilized CD-1 mouse eggs. The mice were backcrossed to C57BL/6J for 6 generations. Upon arrival at The Jackson Laboratory Repository, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Tg(Col1a1*2.3-GFP)1Rowe
Name: transgene insertion 1, David Rowe
MGI accession ID: MGI:4353048
Generation method: Transgenic
Attributes: Reporter
Synonyms: Col1a1-EGFP; Col2.3emd; pOBCol2.3GFP; pOBColGFPemd
Marker symbol: Tg(Col1a1*2.3-GFP)1Rowe
Marker name: transgene insertion 1, David Rowe
Chromosome: UN
Strain of origin: CD-1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP expression is restricted to vertebral bone tissue.
Molecular note: A 2.3 kb promoter fragment of Col1a1 was used to drive expression of GFP. Expression was confirmed by fluorescent microscopy of tail clips from F1 progeny of the three founders. Expression was restricted to vertebral bone tissue.