B6.129-Asic2^tm1Wsh/J

Stock number: 013126
Research areas: Neurobiology Research, Sensorineural Research

Description

Homozygous Asic2 knockout mice display subtle alterations in mechanoreceptor and spiral ganglion function, and are more sensitive to light-induced retinal degeneration. This strain may be helpful in studies of touch sensation.

Detailed description

Homozygous Asic2 (amiloride-sensitive cation channel 1, neuronal (degenerin)) targeted mutation mice display subtle alterations in mechanoreceptor and spiral ganglion function, and are more sensitive to light-induced retinal degeneration. Transcripts and protein are missing for both spliced forms of the gene, as determined by Western and Northern blot analysis of mouse brain. Homozygous mice show a reduced reproductive capacity that is reportedly 25% that of wild-type mice. This strain may be helpful in studies of touch sensation.

Development

Exons 8, 9, and flanking intronic sequences were replaced with a neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.

Allele

Symbol: Asic2^tm1Wsh
Name: targeted mutation 1, Michael J Welsh
MGI accession ID: MGI:2180111
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Asic2
Marker name: acid-sensing (proton-gated) ion channel 2
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A neomycin cassette was inserted to disrupt two exons encoding the second transmembrane domain, which is common to both splice variants, as well as a channel pore. Transcripts were not detected in the brains of homozygous mutant mice. Neither in situ hybridization nor immunostaining detected protein in the large or small dorsal root ganglion neurons of homozygous animals.