Homozygous Asic2 knockout mice display subtle alterations in mechanoreceptor and spiral ganglion function, and are more sensitive to light-induced retinal degeneration. This strain may be helpful in studies of touch sensation.
Michael Welsh, University of Iowa
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Asic2 | acid-sensing (proton-gated) ion channel 2 |
Homozygous Asic2 (amiloride-sensitive cation channel 1, neuronal (degenerin)) targeted mutation mice display subtle alterations in mechanoreceptor and spiral ganglion function, and are more sensitive to light-induced retinal degeneration. Transcripts and protein are missing for both spliced forms of the gene, as determined by Western and Northern blot analysis of mouse brain. Homozygous mice show a reduced reproductive capacity that is reportedly 25% that of wild-type mice. This strain may be helpful in studies of touch sensation.
Exons 8, 9, and flanking intronic sequences were replaced with a neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.
Allele Name | targeted mutation 1, Michael J Welsh |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | ASIC2-; BNC1- |
Gene Symbol and Name | Asic2, acid-sensing (proton-gated) ion channel 2 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 11 |
Molecular Note | A neomycin cassette was inserted to disrupt two exons encoding the second transmembrane domain, which is common to both splice variants, as well as a channel pore. Transcripts were not detected in the brains of homozygous mutant mice. Neither in situ hybridization nor immunostaining detected protein in the large or small dorsal root ganglion neurons of homozygous animals. |
Mutations Made By | Maggie Price, University of Iowa |
When maintained as a live colony, heterozygotes or homozygotes may be bred. Homozygous mice show a reduced reproductive capacity that is reportedly 25% that of wildtype mice.
When using the B6.129-Asic2tm1Wsh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013126 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Accn1<tm1Wsh> |
Frozen Mouse Embryo | B6.129-Asic2<tm1Wsh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Asic2<tm1Wsh>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Asic2<tm1Wsh>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Asic2<tm1Wsh>/J Frozen Embryo | $3373.50 |
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