These floxed mutant mice possess loxP sites flanking the Sox9 gene, This strain may be useful for generating conditional mutations in applications related to endochondral bone formation, limb development and patterning, joint formation, and hair and stem cell differentiation.
Benoit de Crombrugghe, UT MD Anderson Cancer Center
These Sox9flox mutant mice possess a loxP site upstream of exon 2, a neomycin resistance (neo) cassette followed by another loxP site downstream of exon 3 of the SRY-box containing gene 9 gene, Sox9. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, the resulting offspring will have exons 2-3 deleted in the cre-expressing tissue, resulting in inactivation of Sox9 gene function. Breeding these Sox9flox mice to strains that express Cre recombinase ubiquitously results in severe bone defects and perilethality. This strain may be useful for studying cell fate determination including endochondral bone formation, limb development and patterning, joint formation, and hair and stem cell differentiation
A targeting vector was designed to insert a loxP site upstream of exon 2, and a neomycin resistance (neo) cassette followed by another loxP site downstream of exon 3 of the SRY-box containing gene 9 gene, Sox9. The construct was electroporated into 129SvEv embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the donating investigator reported that resulting mice were bred to C57BL/6J (see SNP note below) for at least 8 generations to establish a colony of Sox9flox mice. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
|Allele Name||targeted mutation 2, Benoit de Crombrugghe|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Sox9flox; Sox9loxP|
|Gene Symbol and Name||Sox9, SRY (sex determining region Y)-box 9|
|Strain of Origin||129S7/SvEvBrd-Hprt+|
|Molecular Note||A neomycin resistance gene was inserted downstream of exon 3 and loxP sites flanking exon 2 and neo were introduced.|
When maintaining a live colony, homozygous mice may be bred together.
When using the Sox9flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #013106 in your Materials and Methods section.