This Gadd45b targeted mutation strain may be useful in studies of autoimmune disease.
Binfeng Lu, University of Pittsburgh
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gadd45b | growth arrest and DNA-damage-inducible 45 beta |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Isolated T cells exhibit increased proliferation with activation. Th1 cells from homozygotes are more resistant to activation induced apoptosis. 10 month old homozygotes exhibit mild splenomegaly. Homozygotes are more susceptible to experimental autoimmune encephalomyelitis, with longer duration and severity: 60% of homozygotes die when challenged with a higher dose of heat-inactivated Mycobacterium tuberculosis, compared to no deaths in controls. Immunocomplex deposits develop in the glomeruli of homozygotes.
A targeting vector containing a Neo cassette was used to disrupt exons 3 and 4. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to C57BL/6 for 10 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony. In January 2011, 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed that this strain is segregating at 5 markers likely from a mix of B6 and 129.
Allele Name | targeted mutation 1, Richard A Flavell |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Gadd45b- |
Gene Symbol and Name | Gadd45b, growth arrest and DNA-damage-inducible 45 beta |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 10 |
Molecular Note | Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. The deleted region encoded residues 42 through 161, which make up crucial domains of the protein. |
Mutations Made By | Binfeng Lu, University of Pittsburgh |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129S6-Gadd45btm1Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013101 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gadd45b<tm1Flv> |
Frozen Mouse Embryo | B6;129S6-Gadd45b<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Gadd45b<tm1Flv>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Gadd45b<tm1Flv>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S6-Gadd45b<tm1Flv>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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