STOCK Sox2^tm1.1Lan/J

Stock number: 013093
Product name: Sox2^loxp
Research areas: Developmental Biology Research, Research Tools

Description

These Sox2^flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). This strain may be useful for studying eye and lens development.

Detailed description

These Sox2^flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). When bred to mice that express tissue-specific Cre recombinase, the resulting offspring will have no Sox2 expression in cre-expressing tissues. This strain may be useful for studying eye and lens development.

Homozygous mice are viable and fertile. When maintaining a live colony, homozygous mice may be bred together. It is the experience of colonists at The Jackson Laboratory that homozygous breeding pairs produce few offspring, as does breeding heterozygous females with homozygous males. Therefore, we also maintain this strain by breeding homozygous females with heterozygous males.

Development

A targeting vector was designed to insert a loxP site upstream of the coding exon of the SRY-box containing gene 2 (Sox2) locus, followed by an frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of the exon. The construct was electroporated into 129S/SvEv-derived CMTI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) mice to delete the neo cassette. Progeny were crossed to remove the FLPe transgene, resulting in a colony homozygous for the floxed-Sox2 (Sox2^flox) allele. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Sox2^tm1.1Lan
Name: targeted mutation 1.1, Richard A Lang
MGI accession ID: MGI:4366453
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Sox2
Marker name: SRY (sex determining region Y)-box 2
Chromosome: 3
Strain of origin: Not Specified
Molecular note: A loxP site was inserted usptream of the coding exon and an frt flanked neo cassette with a 3' loxP site was inserted downstream of the exon. The frt flanked neo cassette used for selection was removed by germ line, flp mediated recombination.