These Sox2flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). This strain may be useful for studying eye and lens development.
Richard A. Lang, Cincinnati Children's Hospital
Genetic Background | Generation |
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N2+pN1
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Sox2 | SRY (sex determining region Y)-box 2 |
These Sox2flox mutant mice possess loxP sites flanking the coding exon of SRY-box containing gene 2 (Sox2). When bred to mice that express tissue-specific Cre recombinase, the resulting offspring will have no Sox2 expression in cre-expressing tissues. This strain may be useful for studying eye and lens development.
Homozygous mice are viable and fertile. When maintaining a live colony, homozygous mice may be bred together. It is the experience of colonists at The Jackson Laboratory that homozygous breeding pairs produce few offspring, as does breeding heterozygous females with homozygous males. Therefore, we also maintain this strain by breeding homozygous females with heterozygous males.
A targeting vector was designed to insert a loxP site upstream of the coding exon of the SRY-box containing gene 2 (Sox2) locus, followed by an frt-flanked neomycin resistance (neo) cassette, and a second loxP site downstream of the exon. The construct was electroporated into 129S/SvEv-derived CMTI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Stock No. 005703) mice to delete the neo cassette. Progeny were crossed to remove the FLPe transgene, resulting in a colony homozygous for the floxed-Sox2 (Sox2flox) allele. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.1, Richard A Lang |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Sox2loxP; Sox2tm1.1Apdn |
Gene Symbol and Name | Sox2, SRY (sex determining region Y)-box 2 |
Gene Synonym(s) | |
Strain of Origin | Not Specified |
Chromosome | 3 |
Molecular Note | A loxP site was inserted usptream of the coding exon and an frt flanked neo cassette with a 3' loxP site was inserted downstream of the exon. The frt flanked neo cassette used for selection was removed by germ line, flp mediated recombination. |
Mutations Made By | Richard Lang, Cincinnati Children's Hospital |
When maintaining a live colony, homozygous mice may be bred together. It is the experience of colonists at The Jackson Laboratory that homozygous breeding pairs produce few offspring, as does breeding heterozygous females with homozygous males. Therefore, we also maintain this strain by breeding homozygous females with heterozygous males.
When using the Sox2loxp mouse strain in a publication, please cite the originating article(s) and include JAX stock #013093 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Sox2<tm1.1Lan> |
Frozen Mouse Embryo | STOCK Sox2<tm1.1Lan>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sox2<tm1.1Lan>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sox2<tm1.1Lan>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Sox2<tm1.1Lan>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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