STOCK Dnm1^tm2.1Pdc/J

Stock number: 013073
Research areas: Neurobiology Research, Research Tools

Description

These mice possess loxP sites on either side of exons 2-4 of the Dnm1 (dynamin 1) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of endocytosis.

Detailed description

These mice possess loxP sites on either side of exons 2-4 of the Dnm1 (dynamin 1) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. A widespread block of expression results in a failure to thrive, defects in neurotransmission and lethality by 2 weeks of age. This strain may be useful in studies of endocytosis.

Development

Exons 2-4 were flanked by loxP sites and an FRT-flanked neomycin resistance cassette was placed in intron 1. An embryonic stem (ES) cell line derived from a C57BL/6 x 129S1/SvImJ hybrid was used to create the mutation. Crosses with a C57BL/6 background Flp recombinase strain were used to remove the neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129S1/SvImJ genetic background by the donating laboratory.

Allele

Symbol: Dnm1^tm2.1Pdc
Name: targeted mutation 2.1, Pietro De Camilli
MGI accession ID: MGI:4457321
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Dnm1
Marker name: dynamin 1
Chromosome: 2
Strain of origin: (C57BL/6 x 129S1/SvImJ)F1
Molecular note: Exons 2-4 were flanked by loxP sites and an FRT-flanked neomycin resistance cassette was placed in intron 1. Flp mediated recombination removed the neo cassette leaving exons 2 through 4 floxed.