These mice possess loxP sites on either side of exons 2-4 of the Dnm1 (dynamin 1) gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. This strain may be useful in studies of endocytosis.
Pietro De Camilli, Yale University School of Medicine, HHMI
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Dnm1 | dynamin 1 |
These mice possess loxP sites on either side of exons 2-4 of the Dnm1 (dynamin 1) gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene. A widespread block of expression results in a failure to thrive, defects in neurotransmission and lethality by 2 weeks of age. This strain may be useful in studies of endocytosis.
Exons 2-4 were flanked by loxP sites and an FRT-flanked neomycin resistance cassette was placed in intron 1. An embryonic stem (ES) cell line derived from a C57BL/6 x 129S1/SvImJ hybrid was used to create the mutation. Crosses with a C57BL/6 background Flp recombinase strain were used to remove the neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129S1/SvImJ genetic background by the donating laboratory.
Allele Name | targeted mutation 2.1, Pietro De Camilli |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Dnm1LoxP |
Gene Symbol and Name | Dnm1, dynamin 1 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S1/SvImJ)F1 |
Chromosome | 2 |
Molecular Note | Exons 2-4 were flanked by loxP sites and an FRT-flanked neomycin resistance cassette was placed in intron 1. Flp mediated recombination removed the neo cassette leaving exons 2 through 4 floxed. |
Mutations Made By | Pietro De Camilli, Yale University School of Medicine, HHMI |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Dnm1tm2.1Pdc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013073 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dnm1<tm2.1Pdc> |
Frozen Mouse Embryo | STOCK Dnm1<tm2.1Pdc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dnm1<tm2.1Pdc>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Dnm1<tm2.1Pdc>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Dnm1<tm2.1Pdc>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.