B6;129-Inpp1^tm1Yrk/J

Stock number: 012917

Description

Homozygous Inpp (inositol polyphosphate-1-phosphatase) targeted mutation mice are viable and fertile and have no observable phenotype or physical abnormalities. Although it is unknown whether aberrant transcripts are expressed, the catalytic residues have been eliminated, thus preventing all enzymatic activity. This strain may be useful for further characterization of the targeted gene.

Detailed description

Homozygous Inpp (inositol polyphosphate-1-phosphatase) targeted mutation mice are viable and fertile and have no observable phenotype or physical abnormalities. Although it is unknown whether aberrant transcripts are expressed, the catalytic residues have been eliminated, thus preventing all enzymatic activity. This strain may be useful for further characterization of the targeted gene.

Development

Exons 4 and 5, encoding the catalytic residues were replaced with a neomycin resistance cassette in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Allele

Symbol: Inpp1^tm1Yrk
Name: targeted mutation 1, John D York
MGI accession ID: MGI:4818265
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Inpp1
Marker name: inositol polyphosphate-1-phosphatase
Chromosome: 1
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exons 4 and 5, encoding the catalytic residues, were replaced with a neomycin resistance cassette.