This strain serves as a control for the mutant human lamin A transgenic (R482Q) and may be useful in studies of familial partial lipodystrophy of the Dunnigan type (FPLD2).
Jurrien Dean, NIH / NIDDK
Homozygotes: Not evaluated
Hemizygote: Male mice that are hemizygous for the human wild-type lamin A transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain serves as a control for the mutant human lamin A transgenic (R482Q) and may be useful in studies of familial partial lipodystrophy of the Dunnigan type (FPLD2).
An 11 kb transgenic construct containing a fatty acid binding protein (aP2) promoter, human lamin A cDNA , an SV40 splice site and poly(A) sites was injected into FVB fertilized embryos. Founder mice were bred to FVB/N mice. Upon arrival, mice were bred to FVB/NJ for at least 1 generation to establish the colony.
|Expressed Gene||LMNA, lamin A/C, human|
|Site of Expression|
|Allele Name||transgene insertion, Constantine D Londos|
|Allele Type||Transgenic (Inserted expressed sequence, Humanized sequence)|
|Allele Synonym(s)||Tg(Fabp4-LMNA)#Cdl; transgene insertion, Constantine D Londos|
|Gene Symbol and Name||Tg(Fabp4-LMNA)#Cdl, transgene insertion, Constantine D Londos|
|Promoter||Fabp4, fatty acid binding protein 4, adipocyte, mouse, laboratory|
|Expressed Gene||LMNA, lamin A/C, human|
|Strain of Origin||FVB|
|Molecular Note||The 11 kb transgenic construct contained a fatty acid binding protein (aP2) promoter to drive adipose-specific expression of the human lamin A cDNA followed by an SV40 splice site and poly(A) sites. No line number was given (denoted by #).|
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony. The Donating Investigator has not tried to make this line homozygous.
When using the FVB/N-Tg(Fabp4-LMNA)#Cdl/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32643 in your Materials and Methods section.
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