Male mice that are homozygous for this targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous females are viable, but infertile. No gene product (mRNA or protein) is detected by RT-PCR and Western blot analysis of embyronic fibroblasts from homozygous mice. Homozygous females have reduced ovulation (fewer oocytes recovered from the oviducts after ovulation) and impaired fertilization, resulting in infertility. Homozygotes develop proteoglycan-induced arthritis with greater severity than wildtype mice, with a larger influx of neutrophils into joints, destruction of articular cartilage, erosion of bone and ankylosis. Neutrophil infiltration is also exacerbated in thioglycollate induced peritonitis. The Y chromosome may not have been fixed to the BALB/cAnNCrl genetic background.
A targeting vector containing a PGK-neo cassette was used to disrupt exon 1 of the gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were crossed to BALB/c female mice, and then backcrossed to BALB/cAnNCrl for 14 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony. The Y chromosome may not have been fixed to the BALB/c genetic background.
|Allele Name||targeted mutation 1, Csaba Fulop|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Tnfaip6, tumor necrosis factor alpha induced protein 6|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exon 1 was disrupted by a neomycin selection cassette inserted via homologous recombination. Neither transcript nor protein was detected by RT-PCR and Western blot analysis of homozygous mutant embyronic fibroblasts, with and without Tnfa stimulation.|
|Mutations Made By|| |
Tibor Glant, Rush University Medical Center
When maintaining a live colony, these mice can be bred as heterozygous females and homozygous males. Homozygous females are infertile.
When using the C.129S6-Tnfaip6tm1Cful/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012903 in your Materials and Methods section.
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