B6.129S6-Stat5b^tm1Mam Stat5a^tm2Mam/Mmjax

Stock number: 012900
Product name: Stat5^fl
Research areas: Research Tools

Description

These mutant mice possess loxP sites flanking the signal transducer and activator of transcription 5A and B (Stat5a/Stat5b) loci and may be useful for studying cell proliferation, differentiation and survival in in any given cell type.

Detailed description

Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study cell proliferation, differentiation and survival in in any given cell type.

Heterozygote: Not evaluated

Development

A targeting vector was designed to place Stat5a and Stat5b in a head to toe orientation with no genes between them. A loxP flanked neomycin resistance gene was inserted downstream of Stat5b and a loxP flanked hygromycin resistance gene was inserted downstream of Stat5a. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice for more than 10 generations. Correctly genotyped pups from each generation were selected using marker assisted genome scan analysis (speed congenic). Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Stat5a^tm2Mam
Name: targeted mutation 2, Lothar Hennighausen
MGI accession ID: MGI:3055318
Generation method: Targeted
Marker symbol: Stat5a
Marker name: signal transducer and activator of transcription 5A
Chromosome: 11
Strain of origin: 129S6/SvEvTac
Molecular note: Targeting vectors were designed to insert a floxed hygro downstream of the gene. This allele was generated in conjunction with Stat5b^tm1Mam.

Allele

Symbol: Stat5b^tm1Mam
Name: targeted mutation 1, Lothar Hennighausen
MGI accession ID: MGI:3055319
Generation method: Targeted
Marker symbol: Stat5b
Marker name: signal transducer and activator of transcription 5B
Chromosome: 11
Strain of origin: 129S6/SvEvTac
Molecular note: Targeting vectors were designed to insert a floxed neo downstream of the gene. This allele was generated in conjunction with Stat5a^tm2Mam.