These mutant mice possess loxP sites flanking the signal transducer and activator of transcription 5A and B (Stat5a/Stat5b) loci and may be useful for studying cell proliferation, differentiation and survival in in any given cell type.
Dr. Lothar Hennighausen, National Institutes of Health
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Stat5a | signal transducer and activator of transcription 5A |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Stat5b | signal transducer and activator of transcription 5B |
Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study cell proliferation, differentiation and survival in in any given cell type.
Heterozygote: Not evaluated
A targeting vector was designed to place Stat5a and Stat5b in a head to toe orientation with no genes between them. A loxP flanked neomycin resistance gene was inserted downstream of Stat5b and a loxP flanked hygromycin resistance gene was inserted downstream of Stat5a. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice for more than 10 generations. Correctly genotyped pups from each generation were selected using marker assisted genome scan analysis (speed congenic). Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Allele Name | targeted mutation 2, Lothar Hennighausen |
---|---|
Allele Type | Targeted |
Allele Synonym(s) | Stat5fl; Stat5a/Stat5btm2Mam |
Gene Symbol and Name | Stat5a, signal transducer and activator of transcription 5A |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | Targeting vectors were designed to insert a floxed hygro downstream of the gene. This allele was generated in conjunction with Stat5btm1Mam. |
Allele Name | targeted mutation 1, Lothar Hennighausen |
---|---|
Allele Type | Targeted |
Allele Synonym(s) | Stat5fl; Stat5a/Stat5btm2Mam |
Gene Symbol and Name | Stat5b, signal transducer and activator of transcription 5B |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | Targeting vectors were designed to insert a floxed neo downstream of the gene. This allele was generated in conjunction with Stat5atm2Mam. |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Stat5fl mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32053 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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