Overview

Also Known As:Stat5^fl

These mutant mice possess loxP sites flanking the signal transducer and activator of transcription 5A and B (Stat5a/Stat5b) loci and may be useful for studying cell proliferation, differentiation and survival in in any given cell type.

Donating Investigator

Dr. Lothar Hennighausen, National Institutes of Health

Genetic overview

Genetic Background	Generation

Stat5a^tm2Mam

Allele Type	Gene Symbol	Gene Name
Targeted	Stat5a	signal transducer and activator of transcription 5A

Stat5b^tm1Mam

Allele Type	Gene Symbol	Gene Name
Targeted	Stat5b	signal transducer and activator of transcription 5B

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Research Applications

Research Tools

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Details

Detailed Description

Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene. This mutant mouse strain may be useful in generating conditional mutations to study cell proliferation, differentiation and survival in in any given cell type.

Heterozygote: Not evaluated

Development

A targeting vector was designed to place Stat5a and Stat5b in a head to toe orientation with no genes between them. A loxP flanked neomycin resistance gene was inserted downstream of Stat5b and a loxP flanked hygromycin resistance gene was inserted downstream of Stat5a. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice for more than 10 generations. Correctly genotyped pups from each generation were selected using marker assisted genome scan analysis (speed congenic). Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Cui Y; Riedlinger G; Miyoshi K; Tang W; Li C; Deng CX; Robinson GW; Hennighausen L. 2004. Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation. Mol Cell Biol 24(18):8037-47PubMed: 15340066 MGI: J:92770

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Genetics

Stat5a^tm2Mam

Allele Symbol: Stat5a^tm2Mam

Allele Name	targeted mutation 2, Lothar Hennighausen
Allele Type	Targeted
Allele Synonym(s)	Stat5^fl; Stat5a/Stat5b^tm2Mam
Gene Symbol and Name	Stat5a, signal transducer and activator of transcription 5A
Gene Synonym(s)
Strain of Origin	129S6/SvEvTac
Chromosome	11
Molecular Note	Targeting vectors were designed to insert a floxed hygro downstream of the gene. This allele was generated in conjunction with Stat5b^tm1Mam.

Stat5b^tm1Mam

Allele Symbol: Stat5b^tm1Mam

Allele Name	targeted mutation 1, Lothar Hennighausen
Allele Type	Targeted
Allele Synonym(s)	Stat5^fl; Stat5a/Stat5b^tm2Mam
Gene Symbol and Name	Stat5b, signal transducer and activator of transcription 5B
Gene Synonym(s)
Strain of Origin	129S6/SvEvTac
Chromosome	11
Molecular Note	Targeting vectors were designed to insert a floxed neo downstream of the gene. This allele was generated in conjunction with Stat5a^tm2Mam.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Hematological Research
- Immunology, Inflammation and Autoimmunity Research
- Diabetes and Obesity Research
  - loxP
- Reproductive Biology Research
  - Cre-lox System
- Cre-lox System
  - loxP-flanked Sequences

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Stat5b^tm1Mam/Stat5b^tm1Mam
involves: 129S6/SvEvTac * Black Swiss

normal phenotype

no abnormal phenotype detected
- Normal - homozygotes are indistinguishable from wild-type littermates

Genotype: Stat5a^tm2Mam/Stat5a^tm2Mam Stat5b^tm1Mam/Stat5b⁺
involves: 129S6/SvEvTac

hematopoietic system phenotype

abnormal lymphopoiesis
- impaired NK cell maturation

immune system phenotype

abnormal lymphopoiesis
- impaired NK cell maturation

References

Cui Y; Riedlinger G; Miyoshi K; Tang W; Li C; Deng CX; Robinson GW; Hennighausen L. 2004. Inactivation of Stat5 in mouse mammary epithelium during pregnancy reveals distinct functions in cell proliferation, survival, and differentiation. Mol Cell Biol 24(18):8037-47PubMed: 15340066 MGI: J:92770

Additional - Stat5a^tm2Mam related

Additional - Stat5b^tm1Mam related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Probe:Generic Cre Probe
- Standard PCR:Generic Cre Melt Curve Analysis
- Standard PCR:Stat5a/Stat5b
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Stat5^fl mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32053 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Stat5fl

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Stat5atm2Mam

Allele Symbol: Stat5atm2Mam

Stat5btm1Mam

Allele Symbol: Stat5btm1Mam

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Stat5btm1Mam/Stat5btm1Maminvolves: 129S6/SvEvTac * Black Swiss

normal phenotype

Genotype: Stat5atm2Mam/Stat5atm2Mam Stat5btm1Mam/Stat5b+involves: 129S6/SvEvTac

hematopoietic system phenotype

immune system phenotype

References

Additional - Stat5atm2Mam related

Additional - Stat5btm1Mam related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Stat5^fl

Stat5a^tm2Mam

Allele Symbol: Stat5a^tm2Mam

Stat5b^tm1Mam

Allele Symbol: Stat5b^tm1Mam

Genotype: Stat5b^tm1Mam/Stat5b^tm1Mam
involves: 129S6/SvEvTac * Black Swiss

Genotype: Stat5a^tm2Mam/Stat5a^tm2Mam Stat5b^tm1Mam/Stat5b⁺
involves: 129S6/SvEvTac

Additional - Stat5a^tm2Mam related

Additional - Stat5b^tm1Mam related