STOCK Slc17a6^tm1Lowl/J

Stock number: 012898
Product name: Vglut2^flox
Research areas: Diabetes and Obesity Research, Neurobiology Research, Research Tools

Description

Vglut2^flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene. These mice may be useful for studying the effect of transport, packaging, and release of glutamate from ventromedial hypothalamic (VMH) neurons on hypoglycemia.
Of note, the same Vglut2^flox allele is also available on a C57BL/6J genetic background as Stock No. 033688.

Detailed description

These Vglut2^flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue. These mice may be useful for studying the effect of transport, packaging, and release of glutamate from ventromedial hypothalamic (VMH) neurons on hypoglycemia.

Development

A targeting vector was designed to insert loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene, followed by an frt-flanked kanamycin/neomycin (kan/neo) resistance cassette. The construct was electroporated into W4-derived 129S6/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to FVB or C57BL/6 females. Offspring carrying this Vglut2 allele, were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (Stock No. 003946) mice carrying a flp-recombinase gene to delete the kan/neo cassette, and progeny were crossed to remove the Flp-expressing mutation, resulting in a colony homozygous for the floxed-Vglut2 (Vglut2^flox) allele. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Slc17a6^tm1Lowl
Name: targeted mutation 1, Bradford B Lowell
MGI accession ID: MGI:3771177
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Slc17a6
Marker name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Chromosome: 7
Strain of origin: 129S6/SvEvTac
Molecular note: Lox P sites were inserted upstream and downstream of exon 2. A neo cassette used for selection purposes was removed by flp-mediated recombination.