Vglut2flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene. These mice may be useful for studying the effect of transport, packaging, and release of glutamate from ventromedial hypothalamic (VMH) neurons on hypoglycemia.
Of note, the same Vglut2flox allele is also available on a C57BL/6J genetic background as Stock No. 033688.
Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)
Genetic Background | Generation |
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F?+F16
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Slc17a6 | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
These Vglut2flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue. These mice may be useful for studying the effect of transport, packaging, and release of glutamate from ventromedial hypothalamic (VMH) neurons on hypoglycemia.
A targeting vector was designed to insert loxP sites flanking exon 2 of the solute carrier family 17 (sodium-dependant inorganic phosphate cotransporter), member 6 (Slc17a6 or Vglut2) gene, followed by an frt-flanked kanamycin/neomycin (kan/neo) resistance cassette. The construct was electroporated into W4-derived 129S6/SvEvTac embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to FVB or C57BL/6 females. Offspring carrying this Vglut2 allele, were bred with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (Stock No. 003946) mice carrying a flp-recombinase gene to delete the kan/neo cassette, and progeny were crossed to remove the Flp-expressing mutation, resulting in a colony homozygous for the floxed-Vglut2 (Vglut2flox) allele. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Bradford B Lowell |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Vglut2flox |
Gene Symbol and Name | Slc17a6, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 7 |
Molecular Note | Lox P sites were inserted upstream and downstream of exon 2. A neo cassette used for selection purposes was removed by flp-mediated recombination. |
Mutations Made By | Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard) |
When maintaining a live colony, homozygous mice may be bred together.
When using the Vglut2flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012898 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc17a6<tm1Lowl> |
Frozen Mouse Embryo | STOCK Slc17a6<tm1Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc17a6<tm1Lowl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Slc17a6<tm1Lowl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Slc17a6<tm1Lowl>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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