STOCK Slc32a1^tm1Lowl/J

Stock number: 012897
Product name: Vgat^flox
Research areas: Neurobiology Research, Research Tools

Description

These floxed Slc32a1^tm1Lowl (also commonly referred to as Vgat^flox/flox) mice possess loxP sites flanking exon 2 of the Slc32a1 (or Vgat) gene. These mice may be useful for studying the physiological effect of GABAergic neurotransmission on the regulation of energy balance.

Detailed description

These Vgat^flox/flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 32 (sodium-dependant inorganic phosphate cotransporter), member 1 (Slc32a1 or Vgat) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue. These mice may be useful for studying the physiological effect of GABAergic neurotransmission on the regulation of energy balance.

For example, when crossed to a strain expressing Cre recombinase in agouti-related protein (AgRP) secreting neurons (see Stock No. 012899), this mutant mouse strain may be useful in studies of energy balance.

Development

A targeting vector was designed to insert loxP sites flanking exon 2 of the solute carrier family 32 (sodium-dependant inorganic phosphate cotransporter), member 1 (Slc32a1 or Vgat) gene, followed by an frt-flanked kanamycin/neomycin (kan/neo) resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into 129S6 blastocysts and resulting chimeric males were bred to FVB/N or C57BL/6 females. Offspring carrying this Vglut2 allele, were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (Stock No. 003946) mice carrying a flp-recombinase gene to delete the kan/neo cassette, and progeny were crossed to remove the flp-expressing mutation, resulting in a colony homozygous for the floxed-Vgat (Vgat^flox) allele. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Slc32a1^tm1Lowl
Name: targeted mutation 1, Bradford B Lowell
MGI accession ID: MGI:3815083
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Slc32a1
Marker name: solute carrier family 32 (GABA vesicular transporter), member 1
Chromosome: 2
Strain of origin: 129S6/SvEvTac
Molecular note: LoxP sites were inserted flanking exon 2 and an frt flanked kan/neo cassette was inserted via homologous recombination. The kan/neo cassette was subsequently removed via in vivo Flp mediated recombination.