Overview

Also Known As: Vgat^flox

These floxed Slc32a1^tm1Lowl (also commonly referred to as Vgat^flox/flox) mice possess loxP sites flanking exon 2 of the Slc32a1 (or Vgat) gene. These mice may be useful for studying the physiological effect of GABAergic neurotransmission on the regulation of energy balance.

Donating Investigator

Bradford B. Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Genetic overview

Genetic Background	Generation
	F?+N1F20 (2018-11-30 00:00:00)

Slc32a1^tm1Lowl

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Slc32a1	solute carrier family 32 (GABA vesicular transporter), member 1

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$278.00 Domestic price for female

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Details

Detailed Description

These Vgat^flox/flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 32 (sodium-dependant inorganic phosphate cotransporter), member 1 (Slc32a1 or Vgat) gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 deleted in the cre-expressing tissue. These mice may be useful for studying the physiological effect of GABAergic neurotransmission on the regulation of energy balance.

For example, when crossed to a strain expressing Cre recombinase in agouti-related protein (AgRP) secreting neurons (see Stock No. 012899), this mutant mouse strain may be useful in studies of energy balance.

Development

A targeting vector was designed to insert loxP sites flanking exon 2 of the solute carrier family 32 (sodium-dependant inorganic phosphate cotransporter), member 1 (Slc32a1 or Vgat) gene, followed by an frt-flanked kanamycin/neomycin (kan/neo) resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into 129S6 blastocysts and resulting chimeric males were bred to FVB/N or C57BL/6 females. Offspring carrying this Vglut2 allele, were bred with 129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym/J (Stock No. 003946) mice carrying a flp-recombinase gene to delete the kan/neo cassette, and progeny were crossed to remove the flp-expressing mutation, resulting in a colony homozygous for the floxed-Vgat (Vgat^flox) allele. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Control Suggestions

None Available

Additional Information

Considerations for Choosing Controls

Selected References

Tong Q; Ye CP; Jones JE; Elmquist JK; Lowell BB. 2008. Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance. Nat Neurosci 11(9):998-1000PubMed: 19160495 MGI: J:140858

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Genetics

Slc32a1^tm1Lowl

Allele Symbol: Slc32a1^tm1Lowl

Allele Name	targeted mutation 1, Bradford B Lowell
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)	targeted mutation 1, Bradford B Lowell; Slc32a1^tm1Lowl
Gene Symbol and Name	Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1
Gene Synonym(s)	expressed sequence R75019; R75019; VIAAT; VGAT; R75019; Viaat; vesicular inhibitory amino acid transporter; Vgat; Viaat
Strain of Origin	129S6/SvEvTac
Chromosome	2
Molecular Note	LoxP sites were inserted flanking exon 2 and an frt flanked kan/neo cassette was inserted via homologous recombination. The kan/neo cassette was subsequently removed via in vivo Flp mediated recombination.
Mutations Made By	Bradford Lowell, Beth Israel Deaconess Med Cntr (Harvard)

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
- Receptor Defects
  - GABA receptor
Research Tools
- Diabetes and Obesity Research
  - loxP

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Agrp^tm1(cre)Lowl/Agrp⁺ Slc32a1^tm1Lowl/Slc32a1^tm1Lowl
involves: 129S6/SvEvTac * C57BL/6

adipose tissue phenotype

decreased total body fat amount
- decrease in weight gain on a high fat diet is solely the result of reduced fat accumulation

nervous system phenotype

abnormal inhibitory postsynaptic currents
- absent in AgRP neurons

behavior/neurological phenotype

abnormal food intake
- attenuated response to gherlin induced increase in food intake
- Normal - however, no difference in baseline food intake is detected

hyperactivity
- increased locomotor activity

growth/size/body region phenotype

decreased body weight
- in both males and females on a normal chow diet

decreased susceptibility to diet-induced obesity
- decrease in weight gain when place on a high fat diet

homeostasis/metabolism phenotype

decreased respiratory quotient
- reduced

decreased susceptibility to diet-induced obesity
- decrease in weight gain when place on a high fat diet

increased oxygen consumption
- during the transition from a normal chow diet to a high fat diet there is a larger increase in oxygen consumption compared to controls
- on a normal chow diet

References

Tong Q; Ye CP; Jones JE; Elmquist JK; Lowell BB. 2008. Synaptic release of GABA by AgRP neurons is required for normal regulation of energy balance. Nat Neurosci 11(9):998-1000PubMed: 19160495 MGI: J:140858

Additional References

Bleckert A; Zhang C; Turner MH; Koren D; Berson DM; Park SJH; Demb JB; Rieke F; Wei W; Wong RO. 2018. GABA release selectively regulates synapse development at distinct inputs on direction-selective retinal ganglion cells. Proc Natl Acad Sci U S A 115(51):E12083-E12090PubMed: 30509993 MGI: J:268899

Additional - Slc32a1^tm1Lowl related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Slc32a1^tm1Lowl
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Vgat^flox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012897 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Slc32a1<tm1Lowl>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

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Also Known As: Vgatflox

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Slc32a1tm1Lowl

Allele Symbol: Slc32a1tm1Lowl

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Agrptm1(cre)Lowl/Agrp+ Slc32a1tm1Lowl/Slc32a1tm1Lowlinvolves: 129S6/SvEvTac * C57BL/6

adipose tissue phenotype

nervous system phenotype

behavior/neurological phenotype

growth/size/body region phenotype

homeostasis/metabolism phenotype

References

Additional References

Additional - Slc32a1tm1Lowl related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: Vgat^flox

Slc32a1^tm1Lowl

Allele Symbol: Slc32a1^tm1Lowl

Genotype: Agrp^tm1(cre)Lowl/Agrp⁺ Slc32a1^tm1Lowl/Slc32a1^tm1Lowl
involves: 129S6/SvEvTac * C57BL/6

Additional - Slc32a1^tm1Lowl related