These CD4-IL-17RB transgenic mice may be useful in studies of T cell differentiation and allergic immune response.
Chen Dong, MD Anderson Cancer Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These transgenic mice express the mouse interleukin 17 receptor B under the direction of the mouse CD4 antigen promoter/enhancer. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. The transgene is expressed in all T cells. When treated with IL25, T cells from transgenic mice have reduced numbers of IFN-gamma producing cells, reduced IL4 and IFN-gamma levels and increased IL5 and IL13 levels when compared to wildtype. Transgenic mice challenged with intranasal allergens exhibit higher levels of inflammatory cell infiltration than wildtype controls.
A transgenic construct containing the mouse interleukin 17 receptor B, Il17rb, gene under the control of the mouse CD4 antigen, Cd4, promoter/enhancer with the first intron without the silencer element, was injected into fertilized C57BL/6J mouse eggs. Founder line 5 was subsequently established. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J at least once to establish the colony.
Expressed Gene | Il17rb, interleukin 17 receptor B, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 5, Chen Dong |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Cd4-Il17rb)5Cdon, transgene insertion 5, Chen Dong |
Gene Synonym(s) | |
Promoter | Cd4, CD4 antigen, mouse, laboratory |
Expressed Gene | Il17rb, interleukin 17 receptor B, mouse, laboratory |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | A full length mouse Il17b cDNA was inserted behind the mouse Cd4 promoter/enhancer and the first intron without the silencer element. Three (1, 3 and 5) lines with the highest mRNA expression were studied. |
Mutations Made By | Chen Dong, MD Anderson Cancer Center |
When maintaining a live colony, transgene carrier mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6J inbred mice (Stock No. 000664). The Donating Investigator has not attempted to make the strain homozygous.
When using the C57BL/6-Tg(Cd4-Il17rb)5Cdon/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012895 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Cd4-Il17rb)5Cdon |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Il17rb)5Cdon/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Il17rb)5Cdon/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Il17rb)5Cdon/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Cd4-Il17rb)5Cdon/J | $3373.50 |
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