C57BL/6-Tg(Cd4-Il17rb)5Cdon/J

Stock number: 012895
Research areas: Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

These CD4-IL-17RB transgenic mice may be useful in studies of T cell differentiation and allergic immune response.

Detailed description

These transgenic mice express the mouse interleukin 17 receptor B under the direction of the mouse CD4 antigen promoter/enhancer. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator has not attempted to make the strain homozygous. The transgene is expressed in all T cells. When treated with IL25, T cells from transgenic mice have reduced numbers of IFN-gamma producing cells, reduced IL4 and IFN-gamma levels and increased IL5 and IL13 levels when compared to wildtype. Transgenic mice challenged with intranasal allergens exhibit higher levels of inflammatory cell infiltration than wildtype controls.

Development

A transgenic construct containing the mouse interleukin 17 receptor B, Il17rb, gene under the control of the mouse CD4 antigen, Cd4, promoter/enhancer with the first intron without the silencer element, was injected into fertilized C57BL/6J mouse eggs. Founder line 5 was subsequently established. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J at least once to establish the colony.

Allele

Symbol: Tg(Cd4-Il17rb)5Cdon
Name: transgene insertion 5, Chen Dong
MGI accession ID: MGI:4456140
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Cd4-Il17rb)5Cdon
Marker name: transgene insertion 5, Chen Dong
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: Il17rb,interleukin 17 receptor B,mouse, laboratory
Molecular note: A full length mouse Il17b cDNA was inserted behind the mouse Cd4 promoter/enhancer and the first intron without the silencer element. Three (1, 3 and 5) lines with the highest mRNA expression were studied.