129S-Wls^tm1.1Lan/J

Stock number: 012888
Product name: Wls^¿
Research areas: Developmental Biology Research, Endocrine Deficiency Research, Research Tools

Description

These Wls floxed mutant mice possess loxP site before the ATG start site in the 5' untranslated region of exon 1 and another upstream of exon 2 of the wntless homolog (Drosophila) (Wls) gene. This mutant mouse strain is useful to study Wnt signaling in any organ or tissue that can be targeted with a Cre recombinase.

Detailed description

These Wls floxed mutant mice possess loxP site before the ATG start site in the 5' untranslated region of exon 1 and another upstream of exon 2 of the wntless homolog (Drosophila) (Wls) gene. Mice that are homozygous for this allele are viable, fertile, and normal in size. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 1 deleted in cre-expressing tissues, abolishing gene function. When bred to a strain expressing Cre recombinase in the germline, homozygotes fail to develop mesoderm and are embryonic lethal by E8.5. When bred to a strain expressing Cre recombinase in pancreatic precursors, the mutant mice develop pancreatic hypoplasia. When bred to a strain expressing Cre recombinase in neural crest cells, the mutant mouse strain has severe brain malformations and exhibit postnatal lethality. This mutant mouse strain is useful to study Wnt signaling in any organ or tissue that can be targeted with a Cre recombinase.

Development

A targeting vector was designed to insert a loxP site upstream of the ATG start site in exon 1, followed by an frt-flanked neomycin resistance (neo) cassette and a second loxP site upstream of exon 2 of the wntless homolog (Drosophila) (Wls) gene. The construct was electroporated into 129SvEv-derived "Duffy" embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to mice on a 129 background. Offspring, carrying this floxed-Wls allele, were bred with Flp transgenic mice on a 129 background to delete the neo cassette, and progeny were crossed to remove the frt-flanked transgene, resulting in a colony homozygous for the floxed-Wls allele. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation to establish the colony.



A 48 SNP (single nucleotide polymorphism) panel analysis, with 43 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. One of the 43 markers, on Chromosome 9, was segregating form an unknown source.

Allele

Symbol: Wls^tm1.1Lan
Name: targeted mutation 1.1, Richard A Lang
MGI accession ID: MGI:4452362
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Wls
Marker name: wntless WNT ligand secretion mediator
Chromosome: 3
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was designed to insert a loxP site upstream of the coding sequence in exon 1, followed by an frt-flanked neomycin resistance (neo) cassette and a second loxP site upstream of exon 2 of the wntless homolog (Drosophila) (Wls) gene. Flp mediated recombination removed the neo cassette leaving the coding sequence of exon 1 floxed.