In this strain the entire coding region of the endogenous mouse achaete-scute complex homolog 1 (Ascl1 or Mash1) is replaced with a nuclear localized green fluorescent protein (GFP), abolishing gene function. These mice may be useful for studying the lineage of distinct cell populations, neuronal turnover, and neuronal replacement upon traumatic injury.
Jane E Johnson, University of Texas Southwestern
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Ascl1 | achaete-scute family bHLH transcription factor 1 |
In this strain the entire coding region of the endogenous mouse achaete-scute complex homolog 1 (Ascl1 or Mash1) gene is replaced with a nuclear localized green fluorescent protein (GFP) gene, abolishing gene function. GFP, driven by the Ascl1 promoter sequence, is expressed in all Ascl1-expressing neural progenitor cells in the embryo and the adult brain, including subsets of neurons throughout the central nervous system (CNS) and the peripheral nervous system (PNS), and in neuroendocrine cells in lung and kidney. Homozygotes die within hours of birth due to CNS and PNS disruptions in neural development. Mice heterozygous for the targeted mutation are viable, fertile, and normal in size. These mice may be useful for studying the lineage of distinct cell populations, neuronal turnover, and neuronal replacement upon traumatic injury.
A targeting vector was designed to replace the entire coding region of the Ascl1 gene with a nuclear localized GFP gene and a floxed neomycin (neo) selection cassette. The construct was electroporated into 129/Sv-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6 mice. These mice were then bred to mice containing Cre-recombinase to remove the floxed neo. These mice were then backcrossed at least 6 generations to an outbred ICR strain. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in all Ascl1-expressing neural progenitor cells in the embryo and the adult brain, including subsets of neurons throughout the central nervous system (CNS) and the peripheral nervous system (PNS), and in neuroendocrine cells in lung and kidney. |
Allele Name | targeted mutation 1, Randall R Reed |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Ascl1GFP; Ascl1GFPKI; Mash1deltaGFP |
Gene Symbol and Name | Ascl1, achaete-scute family bHLH transcription factor 1 |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in all Ascl1-expressing neural progenitor cells in the embryo and the adult brain, including subsets of neurons throughout the central nervous system (CNS) and the peripheral nervous system (PNS), and in neuroendocrine cells in lung and kidney. |
Strain of Origin | Not Specified |
Chromosome | 10 |
Molecular Note | The coding region was replaced by a nuclear-localized GFP. GFP expression mimics endogenous expression (unpublished). |
When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony. Homozygotes die shortly after birth due to CNS and PNS disruptions in neural development.
When using the STOCK Ascl1tm1Reed/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012881 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ascl1<tm1Reed> |
Frozen Mouse Embryo | STOCK Ascl1<tm1Reed>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Ascl1<tm1Reed>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Ascl1<tm1Reed>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Ascl1<tm1Reed>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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