B6.Cg-Tg(Syn1-Accn3*)30795-2Wsh/J

Stock number: 012880
Research areas: Neurobiology Research

Description

This mutant mouse line contains a transgene that consists of a hemagglutnin-tagged mouse Accn3 (amiloride-sensitive cation channel 3)cDNA driven by the rat Syn1 (synapsin1) promoter. Hemizygotes demonstrate a deficit in cued and context fear conditioning.

Detailed description

This mutant mouse line contains a transgene that consists of a hemagglutnin-tagged mouse Accn3 (amiloride-sensitive cation channel 3)cDNA driven by the rat Syn1 (synapsin1) promoter. This transgene is highly expressed in neurons. Hemizygotes demonstrate a deficit in cued and context fear conditioning.

Development

A transgenic vector was constructed by combining a double hemagglutinin (HA) epitope tag sequence with mouse Accn3 cDNA, driven by a rat synapsin 1 (Syn1) promoter. The mouse cDNA was modified with a silent mutation (T1233C) to remove an EcoR V restriction site to facilitate vector construction. The vector was introduced into B6SJLF1 fertilized oocytes. This line (30795/2) was backcrossed to C57BL/6 for more than 10 generations by the donating laboratory.

Allele

Symbol: Tg(Syn1-Accn3*)30795-2Wsh
Name: transgene insertion 30795-2, Michael J Welsh
MGI accession ID: MGI:4456468
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: 2HA-mASIC3 30795/2
Marker symbol: Tg(Syn1-Accn3*)30795-2Wsh
Marker name: transgene insertion 30795-2, Michael J Welsh
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: Asic3,acid-sensing (proton-gated) ion channel 3,mouse, laboratory
Molecular note: A transgenic vector combined a double hemagglutinin (HA) epitope tag sequence with mouse Accn3 cDNA, driven by a rat synapsin 1 (Syn1) promoter. The mouse cDNA was modified with a silent mutation (T1233C) to remove an EcoR V restriction site to facilitate vector construction. Lines 30795-3 and 30795-2 were generated.