B6;129P2-Tardbp^{Gt(RRB030)Byg}/J

Stock number: 012850
Research areas: Developmental Biology Research, Neurobiology Research

Description

Tardbp^+/- mice contain a gene trap targeting vector which inserts a β-geo fusion protein into intron 2 of the endogenous TAR DNA-binding Protein 43 (Tardbp) gene, abolishing gene function. These mice may be useful as a lacZ reporter for Tardbp expression in neurodegenerative disorders.

Detailed description

Tardbp^+/- mice contain a gene trap targeting vector which inserts a β-geo fusion protein into intron 2 of the endogenous TAR DNA-binding Protein 43 (Tardbp) gene, abolishing gene function. Heterozygous mice are viable, fertile, and normal in size, while homozygous mice die between E3.5 and E8.5. lacZ expression in heterozygous embryos at E9.5 is restricted to neuroepithelium and is prominent in all neural progenitors from E10.5-12.5. By E12.5 expression is seen in spinal cord progenitors, in differentiated motor neurons, and in the dorsal root ganglia. Adult Tardbp^+/- mice show widespread expression in various regions of the central nervous system. These mice may be useful as a lacZ reporter for Tardbp expression in neurodegenerative disorders.

Development

A "secretory trap" targeting vector was used replace the endogenous TAR DNA-binding Protein 43 (Tardbp) gene with a β-geo fusion protein. The secretory trap contained a splice acceptor and an in-frame β-geo fusion protein (β-galactosidase and neomycin phosphotransferase). This gene trap targeting vector integrated into intron 2 of Tardbp locus and was subsequently electroporated into 129P2/Ola-derived RRB030 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, and chimeric mice were bred with C57BL/6 mice to establish the mutant colony. The donating investigator reports that mutant mice were maintained on this mixed genetic background by breeding heterozygous mice together . Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tardbp^{Gt(RRB030)Byg}
Name: gene trap RRB030, BayGenomics
MGI accession ID: MGI:4126090
Generation method: Gene trapped
Attributes: Reporter; Null/Knockout
Marker symbol: Tardbp
Marker name: TAR DNA binding protein
Marker synonyms: 1190002A23Rik; ALS10; Tdp43; TDP-43
Chromosome: 4
Strain of origin: 129P2/OlaHsd
Site of expression: lacZ expression in heterozygous embryos at E9.5 is restricted to neuroepithelium and is prominent in all neural progenitors from E10.5-12.5. By E12.5 expression is seen in spinal cord progenitors, in differentiated motor neurons, and in the dorsal root ganglia. Adult Tardbp+/- mice show widespread expression in various regions of the central nervous system.
Molecular note: Trapped gene association determined by genome coordinate overlap and strand. The genetrap vector pGT1LXF was inserted in intron 2 at base pair 1226. The resulting gene product includes only the first 65 amino acids of the native protein fused with betaGeo. All functional domains of the native protein are lost.