This ViattlacZ knock out (beta-galactosidase knock in) mutant mouse strain has a homozygous perinatal lethal phenotype, cleft secondary palate, umbilical hernia and may be useful in studies of craniofacial defects.
Brian G. Condie , University of Georgia
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Slc32a1 | solute carrier family 32 (GABA vesicular transporter), member 1 |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous null mice have perinatal lethal phenotype due to respiratory failure. No gene product (mRNA) is detected by RT-PCR analysis of brain tissue from homozygous embryos aged E16.5. Beta-galactosidase staining pattern mimics the endogenous gene expression pattern. Neonate homozygotes exhibit lack of movement, hunched posture, cleft secondary palate, umbilical hernia, and bumps of displaced brown fat deposits in the dorsal cervical area. The Donating Investigator reports that adult heterozygous males on the C57BL/6J background have seizures starting approximately at age 6 months.
A targeting vector containing a loxP site flanked Neo cassette and the lacZ, beta-galactosidase, gene was used to disrupt exon 1, at the start codon. The construct was electroporated into 129X1/SvJ derived GSI-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to B6.Cg-Tg(Tek-cre)12Flv/J (Stock No. 004128) to remove the floxed neo cassette. The mice were then crossed to C57BL/6J to remove the cre recombinase transgene and then backcrossed to C57BL/6J for 14 generations.
Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression | Beta-galactosidase staining pattern mimics the endogenous gene expression pattern. |
Allele Name | targeted mutation 1.1, Brian G Condie |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | ViaatlacZ |
Gene Symbol and Name | Slc32a1, solute carrier family 32 (GABA vesicular transporter), member 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Beta-galactosidase staining pattern mimics the endogenous gene expression pattern. |
Strain of Origin | 129X1/SvJ |
Chromosome | 2 |
Molecular Note | A lacZ and floxed neo cassette were inserted into an NcoI site in exon 1 that includes the start site via homologous recombination. The neo was removed by Cre mediated recombination. RT-PCR confirmed the absence of transcript in homozygous mice. |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes have a perinatal lethal phenotype. The Donating Investigator reports that adult heterozygous males on the C57BL/6J background have seizures starting approximately at age 6 months.
When using the B6.129X1(Cg)-Slc32a1tm1.1Bgc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012843 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Slc32a1<tm1.1Bgc> |
Frozen Mouse Embryo | B6.129X1(Cg)-Slc32a1<tm1.1Bgc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Slc32a1<tm1.1Bgc>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1(Cg)-Slc32a1<tm1.1Bgc>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1(Cg)-Slc32a1<tm1.1Bgc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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