B6;129X1-Park7^tm1Cai/Mmjax

Stock number: 012835
Research areas: Neurobiology Research

Description

These Park7 knockout mice exhibit non-progressive hypokinesia, progressive gait abnormalities, decreased grip strength, loss of body weight and may be useful in studies of Parkinson's disease.

Detailed description

Homozygote: Mice that are homozygous for the targeted mutation exhibit non-progressive hypokinesia at 5 months of age and progressive gait abnormalities (stride uniformity, hind base displacement) beginning at two months of age. Mice over a year old develop decreased grip strength, loss of body weight and severe gait abnormalities. Although early onset progressive motor deficits occur in these mice, no pathological changes are observed in the nigrostriatal system, spinal motor system or muscles. This mutant mouse strain may be useful in studies of Parkinson's disease.

Heterozygote: Not evaluated

Development

A targeting vector containing a neomycin resistance cassette was used to disrupt exon 2. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and maintained by intercross. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Park7^tm1Cai
Name: targeted mutation 1, Hubaibin Cai
MGI accession ID: MGI:3522482
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Park7
Marker name: Parkinson disease (autosomal recessive, early onset) 7
Marker synonyms: CAP1; DJ1; DJ-1; GATD2; HEL-S-67p; SP22
Chromosome: 4
Strain of origin: 129X1/SvJ
Molecular note: The first coding exon was replaced with a selection marker flanked with loxp sites. QPCR and Western blot analysis confirmed the absence of gene products.