These Park7 knockout mice exhibit non-progressive hypokinesia, progressive gait abnormalities, decreased grip strength, loss of body weight and may be useful in studies of Parkinson's disease.
Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA)
Homozygote: Mice that are homozygous for the targeted mutation exhibit non-progressive hypokinesia at 5 months of age and progressive gait abnormalities (stride uniformity, hind base displacement) beginning at two months of age. Mice over a year old develop decreased grip strength, loss of body weight and severe gait abnormalities. Although early onset progressive motor deficits occur in these mice, no pathological changes are observed in the nigrostriatal system, spinal motor system or muscles. This mutant mouse strain may be useful in studies of Parkinson's disease.
Heterozygote: Not evaluated
A targeting vector containing a neomycin resistance cassette was used to disrupt exon 2. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and maintained by intercross. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
|Allele Name||targeted mutation 1, Hubaibin Cai|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Park7, Parkinson disease (autosomal recessive, early onset) 7|
|Strain of Origin||129X1/SvJ|
|Molecular Note||The first coding exon was replaced with a selection marker flanked with loxp sites. QPCR and Western blot analysis confirmed the absence of gene products.|
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6;129X1-Park7tm1Cai/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32090 in your Materials and Methods section.