Overview

These Park7 knockout mice exhibit non-progressive hypokinesia, progressive gait abnormalities, decreased grip strength, loss of body weight and may be useful in studies of Parkinson's disease.

Donating Investigator

Huaibin Cai, National Institutes of Health / National Institute on Aging (NIH/NIA)

Genetic overview

Genetic Background	Generation

Park7^tm1Cai

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Park7	Parkinson disease (autosomal recessive, early onset) 7

View Genetics

Research Applications

Neurobiology Research

View All Research Applications

Details

Detailed Description

Homozygote: Mice that are homozygous for the targeted mutation exhibit non-progressive hypokinesia at 5 months of age and progressive gait abnormalities (stride uniformity, hind base displacement) beginning at two months of age. Mice over a year old develop decreased grip strength, loss of body weight and severe gait abnormalities. Although early onset progressive motor deficits occur in these mice, no pathological changes are observed in the nigrostriatal system, spinal motor system or muscles. This mutant mouse strain may be useful in studies of Parkinson's disease.

Heterozygote: Not evaluated

Development

A targeting vector containing a neomycin resistance cassette was used to disrupt exon 2. The construct was electroporated into 129X1/SvJ derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice and maintained by intercross. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Chandran JS; Lin X; Zapata A; Hoke A; Shimoji M; Moore SO; Galloway MP; Laird FM; Wong PC; Price DL; Bailey KR; Crawley JN; Shippenberg T; Cai H. 2008. Progressive behavioral deficits in DJ-1-deficient mice are associated with normal nigrostriatal function. Neurobiol Dis 29(3):505-14PubMed: 18187333 MGI: J:141628

View All References

Genetics

Park7^tm1Cai

Allele Symbol: Park7^tm1Cai

Allele Name	targeted mutation 1, Hubaibin Cai
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Park7, Parkinson disease (autosomal recessive, early onset) 7
Gene Synonym(s)
Strain of Origin	129X1/SvJ
Chromosome	4
Molecular Note	The first coding exon was replaced with a selection marker flanked with loxp sites. QPCR and Western blot analysis confirmed the absence of gene products.

Disease/Phenotype

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Parkinson's disease 7

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Ataxia (Movement) Defects
- Parkinson's Disease
  - Park7 (DJ-1) mutants

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Park7^tm1Cai/Park7^tm1Cai
involves: 129X1/SvJ * C57BL/6

behavior/neurological phenotype

abnormal gait
- defects are more severe in two year old mice
- two month old mice have defects in stride uniformity and hind base displacement

abnormal motor learning
- 16-month old mice have enhanced motor learning over 3 days with 62.3% increase in latency time to fall compared to just a 34.6% increase for wild-type controls

decreased grip strength
- mice at 16 months of age, but not 2 months of age, have a significant decrease in foregrip strength

hypoactivity
- mice at 5 and 14 months of age have decreased activity

short stride length
- dopamine treatment failed to increase stride length as it did for wild-type mice
- Normal - this defect in stride length is not observed in 2-month mice
- two-year old mice have a mean decrease in stride length with a mean of 5.35 cm vs. 5.9 for controls

growth/size/body region phenotype

weight loss
- Normal - mice weigh less than controls starting around 1 year in age

References

Chandran JS; Lin X; Zapata A; Hoke A; Shimoji M; Moore SO; Galloway MP; Laird FM; Wong PC; Price DL; Bailey KR; Crawley JN; Shippenberg T; Cai H. 2008. Progressive behavioral deficits in DJ-1-deficient mice are associated with normal nigrostriatal function. Neurobiol Dis 29(3):505-14PubMed: 18187333 MGI: J:141628

Additional - Park7^tm1Cai related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Park7
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129X1-Park7^tm1Cai/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32090 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

Credit for PRODUCTS or SERVICES

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.

Animal Care and Use for SERVICES

Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Park7tm1Cai

Allele Symbol: Park7tm1Cai

Disease Terms

Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Park7tm1Cai/Park7tm1Caiinvolves: 129X1/SvJ * C57BL/6

behavior/neurological phenotype

growth/size/body region phenotype

References

Additional - Park7tm1Cai related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Park7^tm1Cai

Allele Symbol: Park7^tm1Cai

Genotype: Park7^tm1Cai/Park7^tm1Cai
involves: 129X1/SvJ * C57BL/6

Additional - Park7^tm1Cai related