These mice express cre under the direction of the Fev promoter. They may be useful for inducing cre-mediated recombination in serotonergic neurons.
Evan Deneris, Case Western Reserve University
Genetic Background | Generation |
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N5pN1+N2F14
|
Allele Type |
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Transgenic (Recombinase-expressing) |
Starting at:
$255.00 Domestic price for female 4-week |
333.51 Domestic price for breeder pair |
Mice hemizygous for the ePet-Cre transgene are viable and fertile, with the FEV (ePet or Pet1 ETS oncogene family) promoter sequence directing expression of Cre recombinase in all mid-hindbrain serotonin (5HT)-synthesizing neurons and their postmitotic precursors. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for inducing cre-mediated recombination in serotonergic neurons.
The Fev-Cre transgene was designed with (5' to 3') the FEV (ePet or Pet1 ETS oncogene family) gene promoter, a β-globin minimal promoter, Cre-recombinase, and a simian virus 40 polyadenylation (polyA) sequence. This transgene was injected into (C57BL/6 x SJL)F2 oocytes and founder mice were bred to C57BL/6 mice. These mice were backcrossed with C57BL/6 inbred mice for at least 5 generations. Upon arrival at The Jackson Laboratory, ePet-Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase is expressed in all mid-hindbrain serotonin (5HT)-synthesizing neurons and their postmitotic precursors. |
Allele Name | transgene insertion 1, Evan S Deneris |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | ePet::Cre; ePet1-Cre; ePet-cre |
Gene Symbol and Name | Tg(Fev-cre)1Esd, transgene insertion 1, Evan S Deneris |
Gene Synonym(s) | |
Promoter | Fev, FEV (ETS oncogene family), mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase is expressed in all mid-hindbrain serotonin (5HT)-synthesizing neurons and their postmitotic precursors. |
Strain of Origin | (C57BL/6 x SJL)F2 |
Chromosome | UN |
Molecular Note | Cre recombinase was placed under control of the FEV (ETS oncogene family) promoter to be expressed in a serotonergic-specific manner. |
Mutations Made By | Evan Deneris, Case Western Reserve University |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
When using the ePet-cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #012712 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(Fev-cre)1Esd |
Frozen Mouse Embryo | B6.Cg-Tg(Fev-cre)1Esd/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Fev-cre)1Esd/J | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Fev-cre)1Esd/J | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Fev-cre)1Esd/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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