B6.Cg-Tg(Fev-cre)1Esd/J

Stock number: 012712
Product name: ePet-cre
Research areas: Neurobiology Research

Description

These mice express cre under the direction of the Fev promoter. They may be useful for inducing cre-mediated recombination in serotonergic neurons.

Detailed description

Mice hemizygous for the ePet-Cre transgene are viable and fertile, with the FEV (ePet or Pet1 ETS oncogene family) promoter sequence directing expression of Cre recombinase in all mid-hindbrain serotonin (5HT)-synthesizing neurons and their postmitotic precursors. When these transgenic mice are bred with mice containing a loxP-flanked sequence, Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for inducing cre-mediated recombination in serotonergic neurons.

Development

The Fev-Cre transgene was designed with (5' to 3') the FEV (ePet or Pet1 ETS oncogene family) gene promoter, a β-globin minimal promoter, Cre-recombinase, and a simian virus 40 polyadenylation (polyA) sequence. This transgene was injected into (C57BL/6 x SJL)F2 oocytes and founder mice were bred to C57BL/6 mice. These mice were backcrossed with C57BL/6 inbred mice for at least 5 generations. Upon arrival at The Jackson Laboratory, ePet-Cre transgenic mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Tg(Fev-cre)1Esd
Name: transgene insertion 1, Evan S Deneris
MGI accession ID: MGI:3696982
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Fev-cre)1Esd
Marker name: transgene insertion 1, Evan S Deneris
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase is expressed in all mid-hindbrain serotonin (5HT)-synthesizing neurons and their postmitotic precursors.
Molecular note: Cre recombinase was placed under control of the FEV (ETS oncogene family) promoter to be expressed in a serotonergic-specific manner.