Overview

Also Known As: CCK-ires-Cre

The Cck-IRES-Cre knock-in allele has an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, Cre recombinase expression is directed to Cck-expressing cells (cholecystokinin positive neurons) by the endogenous promoter/enhancer elements of the cholecystokinin locus.

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	F?+F19 (2019-03-18 00:00:00)

Cck^{tm1.1(cre)Zjh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Cck	cholecystokinin

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$255.00 Domestic price for female 4-week

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Details

Detailed Description

The Cck-IRES-Cre allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, cre expression is directed by the endogenous Cck promoter/enhancer elements. When Cck-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cck-expressing cells in the offspring. Cck expression from the Cck-IRES-Cre allele has not been evaluated. Additional phenotype information described below.

In 2010, the donating investigator reported Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Cck expression pattern with highly efficient recombination). They report Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex. The donating investigator did not examine cre expression in tissues other than brain (October 2012). Cck expression from the Cck-IRES-Cre allele was not evaluated. They also reported that homozygous mice are viable and fertile.

For characterization information, see images at the Allen Institute for Brain Science website (Cck-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Cck^{tm1.1(cre)Zjh}). This same information would also be found searching the MGI Recombinase Activity database.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background (N10); see Stock No. 005703) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory Repository. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

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Genetics

Cck^{tm1.1(cre)Zjh}

Allele Symbol: Cck^{tm1.1(cre)Zjh}

Allele Name	targeted mutation 1.1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cck^{tm1.1(cre)Zjh}; targeted mutation 1.1, Z Josh Huang
Gene Symbol and Name	Cck, cholecystokinin
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex.
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	9
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed).
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: Cre-lox System
- Neurobiology Research
  - cell marker
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cck^{tm1.1(cre)Zjh}/Cck⁺
involves: 129S4/SvJae * C57BL/6 * SJL

mammalian phenotype

no phenotypic analysis

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional References

Lee S; Kim JH. 2019. Basal Forebrain Cholinergic-induced Activation of Cholecystokinin Inhibitory Neurons in the Basolateral Amygdala. Exp Neurobiol 28(3):320-328PubMed: 31308792 MGI: J:278865

Additional - Cck^{tm1.1(cre)Zjh} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Cck^{tm1.1(cre)Zjh}
- Standard PCR:Cck^{tm1.1(cre)Zjh}
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the CCK-ires-Cre mouse strain in a publication, please cite the originating article(s) and include JAX stock #012706 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Cck<tm1.1(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo	STOCK Cck<tm1.1(cre)Zjh>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Cck<tm1.1(cre)Zjh>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	STOCK Cck<tm1.1(cre)Zjh>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	STOCK Cck<tm1.1(cre)Zjh>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: CCK-ires-Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Ccktm1.1(cre)Zjh

Allele Symbol: Ccktm1.1(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Ccktm1.1(cre)Zjh/Cck+involves: 129S4/SvJae * C57BL/6 * SJL

mammalian phenotype

References

Additional References

Additional - Ccktm1.1(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cck^{tm1.1(cre)Zjh}

Allele Symbol: Cck^{tm1.1(cre)Zjh}

Genotype: Cck^{tm1.1(cre)Zjh}/Cck⁺
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Cck^{tm1.1(cre)Zjh} related