JAX Mouse Strain Datasheet - 012706

STOCK Cck^{tm1.1(cre)Zjh}/J

Stock No:: 012706 |; CCK-ires-Cre

Targeted Mutation

Repository Live

Overview

Also Known As: CCK-ires-Cre

The Cck-IRES-Cre knock-in allele has an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, Cre recombinase expression is directed to Cck-expressing cells (cholecystokinin positive neurons) by the endogenous promoter/enhancer elements of the cholecystokinin locus.

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation
	F?+F15 (17-NOV-17)

Cck^{tm1.1(cre)Zjh}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Cck	cholecystokinin

View Genetics

Research Applications

Neurobiology Research
Research Tools

View All Research Applications

Base Price

Starting at:

$255.00 Domestic price for female

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Details

Detailed Description

The Cck-IRES-Cre allele harbors an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, cre expression is directed by the endogenous Cck promoter/enhancer elements. When Cck-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cck-expressing cells in the offspring. Cck expression from the Cck-IRES-Cre allele has not been evaluated. Additional phenotype information described below.

In 2010, the donating investigator reported Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Cck expression pattern with highly efficient recombination). They report Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex. The donating investigator did not examine cre expression in tissues other than brain (October 2012). Cck expression from the Cck-IRES-Cre allele was not evaluated. They also reported that homozygous mice are viable and fertile.

For characterization information, see images at the Allen Institute for Brain Science website (Cck-IRES-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Cck^{tm1.1(cre)Zjh}). This same information would also be found searching the MGI Recombinase Activity database.

Development

A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background (N10); see Stock No. 005703) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory Repository. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex.

Control Suggestions

Approximate Controls

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. MGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. PubMed: 21943598 MGI: J:177261

View All References

Genetics

Cck^{tm1.1(cre)Zjh}

Allele Symbol: Cck^{tm1.1(cre)Zjh}

Allele Name	targeted mutation 1.1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cck-IRES-Cre
Gene Symbol and Name	Cck, cholecystokinin
Gene Synonym(s)
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex.
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	9
Molecular Note	A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed).
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue
Research Tools
- Cre-lox System
  - Cre Recombinase Expression
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: neurons
- Neurobiology Research
  - cell marker

Genotype: cre related

Research Tools
- Cre-lox System
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Cck^{tm1.1(cre)Zjh}/Cck⁺
involves: 129S4/SvJae * C57BL/6 * SJL

no phenotypic analysis

no phenotypic analysis

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data Submission :. MGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013. PubMed: 21943598 MGI: J:177261

Additional - Cck^{tm1.1(cre)Zjh} related

Allen Institute for Brain Science. 2009. Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS) MGI Direct Data Submission :. MGI: J:146821
Basu J; Zaremba JD; Cheung SK; Hitti FL; Zemelman BV; Losonczy A; Siegelbaum SA. 2016. Gating of hippocampal activity, plasticity, and memory by entorhinal cortex long-range inhibition. Science 351(6269):aaa5694. PubMed: 26744409 MGI: J:228422
Campbell JN; Macosko EZ; Fenselau H; Pers TH; Lyubetskaya A; Tenen D; Goldman M; Verstegen AM; Resch JM; McCarroll SA; Rosen ED; Lowell BB; Tsai LT. 2017. A molecular census of arcuate hypothalamus and median eminence cell types. Nat Neurosci 20(3):484-496. PubMed: 28166221 MGI: J:243793
Chung S; Weber F; Zhong P; Tan CL; Nguyen TN; Beier KT; Hormann N; Chang WC; Zhang Z; Do JP; Yao S; Krashes MJ; Tasic B; Cetin A; Zeng H; Knight ZA; Luo L; Dan Y. 2017. Identification of preoptic sleep neurons using retrograde labelling and gene profiling. Nature :. PubMed: 28514446 MGI: J:241134
Economo MN; Hansen KR; Wachowiak M. 2016. Control of Mitral/Tufted Cell Output by Selective Inhibition among Olfactory Bulb Glomeruli. Neuron 91(2):397-411. PubMed: 27346531 MGI: J:256385
Flak JN; Patterson CM; Garfield AS; D'Agostino G; Goforth PB; Sutton AK; Malec PA; Wong JM; Germani M; Jones JC; Rajala M; Satin L; Rhodes CJ; Olson DP; Kennedy RT; Heisler LK; Myers MG Jr. 2014. Leptin-inhibited PBN neurons enhance responses to hypoglycemia in negative energy balance. Nat Neurosci 17(12):1744-50. PubMed: 25383904 MGI: J:219525
Garfield AS; Patterson C; Skora S; Gribble FM; Reimann F; Evans ML; Myers MG Jr; Heisler LK. 2012. Neurochemical characterization of body weight-regulating leptin receptor neurons in the nucleus of the solitary tract. Endocrinology 153(10):4600-7. PubMed: 22869346 MGI: J:191291
Garfield AS; Shah BP; Madara JC; Burke LK; Patterson CM; Flak J; Neve RL; Evans ML; Lowell BB; Myers MG Jr; Heisler LK. 2014. A parabrachial-hypothalamic cholecystokinin neurocircuit controls counterregulatory responses to hypoglycemia. Cell Metab 20(6):1030-7. PubMed: 25470549 MGI: J:219489
Gee JM; Smith NA; Fernandez FR; Economo MN; Brunert D; Rothermel M; Morris SC; Talbot A; Palumbos S; Ichida JM; Shepherd JD; West PJ; Wachowiak M; Capecchi MR; Wilcox KS; White JA; Tvrdik P. 2014. Imaging Activity in Neurons and Glia with a Polr2a-Based and Cre-Dependent GCaMP5G-IRES-tdTomato Reporter Mouse. Neuron :. PubMed: 25155958 MGI: J:212315
Genetic Resource Science at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2012 Direct Data Submission from Genetic Resource Science :. MGI: J:184579
Harris JA; Hirokawa KE; Sorensen SA; Gu H; Mills M; Ng LL; Bohn P; Mortrud M; Ouellette B; Kidney J; Smith KA; Dang C; Sunkin S; Bernard A; Oh SW; Madisen L; Zeng H. 2014. Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits 8:76. PubMed: 25071457 MGI: J:220523
He M; Tucciarone J; Lee S; Nigro MJ; Kim Y; Levine JM; Kelly SM; Krugikov I; Wu P; Chen Y; Gong L; Hou Y; Osten P; Rudy B; Huang ZJ. 2016. Strategies and Tools for Combinatorial Targeting of GABAergic Neurons in Mouse Cerebral Cortex. Neuron :. PubMed: 27618674 MGI: J:235123
Kim Y; Yang GR; Pradhan K; Venkataraju KU; Bota M; Garcia Del Molino LC; Fitzgerald G; Ram K; He M; Levine JM; Mitra P; Huang ZJ; Wang XJ; Osten P. 2017. Brain-wide Maps Reveal Stereotyped Cell-Type-Based Cortical Architecture and Subcortical Sexual Dimorphism. Cell 171(2):456-469.e22. PubMed: 28985566 MGI: J:250011
Maksimovic S; Nakatani M; Baba Y; Nelson AM; Marshall KL; Wellnitz SA; Firozi P; Woo SH; Ranade S; Patapoutian A; Lumpkin EA. 2014. Epidermal Merkel cells are mechanosensory cells that tune mammalian touch receptors. Nature 509(7502):617-21. PubMed: 24717432 MGI: J:213252
Martersteck EM; Hirokawa KE; Evarts M; Bernard A; Duan X; Li Y; Ng L; Oh SW; Ouellette B; Royall JJ; Stoecklin M; Wang Q; Zeng H; Sanes JR; Harris JA. 2017. Diverse Central Projection Patterns of Retinal Ganglion Cells. Cell Rep 18(8):2058-2072. PubMed: 28228269 MGI: J:247473
Medrihan L; Sagi Y; Inde Z; Krupa O; Daniels C; Peyrache A; Greengard P. 2017. Initiation of Behavioral Response to Antidepressants by Cholecystokinin Neurons of the Dentate Gyrus. Neuron 95(3):564-576.e4. PubMed: 28735749 MGI: J:256106
Roman CW; Derkach VA; Palmiter RD. 2016. Genetically and functionally defined NTS to PBN brain circuits mediating anorexia. Nat Commun 7:11905. PubMed: 27301688 MGI: J:239889
Rothermel M; Brunert D; Zabawa C; Diaz-Quesada M; Wachowiak M. 2013. Transgene expression in target-defined neuron populations mediated by retrograde infection with adeno-associated viral vectors. J Neurosci 33(38):15195-206. PubMed: 24048849 MGI: J:231995
Ryan PJ; Ross SI; Campos CA; Derkach VA; Palmiter RD. 2017. Oxytocin-receptor-expressing neurons in the parabrachial nucleus regulate fluid intake. Nat Neurosci 20(12):1722-1733. PubMed: 29184212 MGI: J:256724
Severson KS; Xu D; Van de Loo M; Bai L; Ginty DD; O''Connor DH. 2017. Active Touch and Self-Motion Encoding by Merkel Cell-Associated Afferents. Neuron 94(3):666-676.e9. PubMed: 28434802 MGI: J:253223
Shekhar K; Lapan SW; Whitney IE; Tran NM; Macosko EZ; Kowalczyk M; Adiconis X; Levin JZ; Nemesh J; Goldman M; McCarroll SA; Cepko CL; Regev A; Sanes JR. 2016. Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics. Cell 166(5):1308-1323.e30. PubMed: 27565351 MGI: J:236391
Tien NW; Soto F; Kerschensteiner D. 2017. Homeostatic Plasticity Shapes Cell-Type-Specific Wiring in the Retina. Neuron 94(3):656-665.e4. PubMed: 28457596 MGI: J:253559
Vogel E; Krabbe S; Grundemann J; Wamsteeker Cusulin JI; Luthi A. 2016. Projection-Specific Dynamic Regulation of Inhibition in Amygdala Micro-Circuits. Neuron 91(3):644-51. PubMed: 27497223 MGI: J:253917
Zhu Y; Xu J; Hauswirth WW; DeVries SH. 2014. Genetically targeted binary labeling of retinal neurons. J Neurosci 34(23):7845-61. PubMed: 24899708 MGI: J:211630

Pricing & Availability

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Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Cck<tm1.1(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: CCK-ires-Cre

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Ccktm1.1(cre)Zjh

Allele Symbol: Ccktm1.1(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Genotype: cre related

Mammalian Phenotype Terms by Genotype

Genotype: Ccktm1.1(cre)Zjh/Cck+involves: 129S4/SvJae * C57BL/6 * SJL

no phenotypic analysis

References

Additional - Ccktm1.1(cre)Zjh related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cck^{tm1.1(cre)Zjh}

Allele Symbol: Cck^{tm1.1(cre)Zjh}

Genotype: Cck^{tm1.1(cre)Zjh}/Cck⁺
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Cck^{tm1.1(cre)Zjh} related