Homozygotes: Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. CD4+ T cells exhibit increased interferon gamma production in vivo when stimulated with low anti-CD3 concentrations; CD8+ T cells exhibit increased interferon gamma production, but only when stimulated with higher antibody concentrations, in both cases, T cell proliferation is not effected. OVA primed CD8+ T cells produce 5-10 fold more interferon gamma than controls. T cell clonal expansion is augmented and cytolysis is increased in mutant antigen-specific CD8+ T cells. Unlike experimental immune encephalitis (EAE) resistant 129S4/SvJae mice, immunized mutant mice on the 129S4/SvJae background develop severe EAE with early onset and rapid progression. Mice exhibit resistance to Leishmania mexicana and a decrease in IL4 producing cells, suggesting an impaired Th2 response. This mutant mouse strain may be useful in studies of T cell regulation, activation and tolerance.

Heterozygote: no phenotype

Immunized CD274 (PD-L1) knockout mice develop severe EAE. Mice exhibit resistance to Leishmania mexicana and a decrease in IL4 producing cells (suggesting an impaired Th2 response) and increased interferon gamma production under certain conditions. This strain may be useful in studies of T cell regulation, activation and tolerance.

This strain is hosted by NIH's MMRRC, which partners with JAX for the distribution of this and other strains. For additional information and to purchase, please visit the MMRRC site.