129S4/SvJae-Cd274^tm2Shr Pdcd1lg2^tm4Shr/Mmjax

Stock number: 012674
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

These targeted mutant mice possess insertions in the IgV exons of Pdcd1lg2 (programmed cell death 1 ligand 2) and Cd274, which affects lymphocyte activation and may be useful in studies of T cell regulation, activation and tolerance.

Detailed description

Mice that are homozygous for both targeted alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice exhibit defects in lymphocyte activation. This mutant mouse strain may be useful in studies of T cell regulation, activation and tolerance.

Development

The Cd274^tm2Shr targeting vector was used to re-target Pdcd1lg2 by replacing the IgV exon of with a hygromycin resistance cassette. The original Cd274^tm2Shr targeting vector contains neomycin resistance and herpes simplex virus thymidine kinase genes that disrupt the signal exon and two-thirds of the IgV exon. The construct was electroporated into heterozygous Cd274^tm2Shr 129S4/SvJae J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJae mice.

Allele

Symbol: Cd274^tm2Shr
Name: targeted mutation 2, Arlene H Sharpe
MGI accession ID: MGI:3050804
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Cd274
Marker name: CD274 antigen
Chromosome: 19
Strain of origin: 129S4/SvJae
Molecular note: A neomycin resistance gene replaced the signal exon and two-thirds of the IgV exon. Southern blot confirmed recombination.

Allele

Symbol: Pdcd1lg2^tm4Shr
Name: targeted mutation 4, Arlene H Sharpe
MGI accession ID: MGI:4456974
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Pdcd1lg2
Marker name: programmed cell death 1 ligand 2
Chromosome: 19
Strain of origin: 129S4/SvJae
Molecular note: A targeting vector was designed to replace the the IgV exon with a hygromycin resistance gene in ES cells containing the Cd274^tm2Shr allele in which the CD274 antigen signal and two-thirds of the IgV exon were replaced with a neomycin cassette.