These targeted mutant mice possess insertions in the IgV exons of Pdcd1lg2 (programmed cell death 1 ligand 2) and Cd274, which affects lymphocyte activation and may be useful in studies of T cell regulation, activation and tolerance.
Arlene H Sharpe, Harvard Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Cd274 | CD274 antigen |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Pdcd1lg2 | programmed cell death 1 ligand 2 |
Mice that are homozygous for both targeted alleles are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Mice exhibit defects in lymphocyte activation. This mutant mouse strain may be useful in studies of T cell regulation, activation and tolerance.
The Cd274tm2Shr targeting vector was used to re-target Pdcd1lg2 by replacing the IgV exon of with a hygromycin resistance cassette. The original Cd274tm2Shr targeting vector contains neomycin resistance and herpes simplex virus thymidine kinase genes that disrupt the signal exon and two-thirds of the IgV exon. The construct was electroporated into heterozygous Cd274tm2Shr 129S4/SvJae J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJae mice.
Allele Name | targeted mutation 2, Arlene H Sharpe |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PD-L1- |
Gene Symbol and Name | Cd274, CD274 antigen |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 19 |
Molecular Note | A neomycin resistance gene replaced the signal exon and two-thirds of the IgV exon. Southern blot confirmed recombination. |
Mutations Made By | Arlene Sharpe, Harvard Medical School |
Allele Name | targeted mutation 4, Arlene H Sharpe |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | PDL1/L2 |
Gene Symbol and Name | Pdcd1lg2, programmed cell death 1 ligand 2 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 19 |
Molecular Note | A targeting vector was designed to replace the the IgV exon with a hygromycin resistance gene in ES cells containing the Cd274tm2Shr allele in which the CD274 antigen signal and two-thirds of the IgV exon were replaced with a neomycin cassette. |
Mutations Made By | Arlene Sharpe, Harvard Medical School |
While maintaining a live colony, these mice may be bred as homozygous for both targeted alleles.
When using the 129S4/SvJae-Cd274tm2Shr Pdcd1lg2tm4Shr/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32239 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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