129S.129-Lrp5^tm1Grw/J

Stock number: 012668
Research areas: Developmental Biology Research, Internal/Organ Research

Description

Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).

Detailed description

Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. Expression of the targeted gene is not detectable in Western blots of liver, kidney and bone. Although the targeting construct was intended to generate a lacZ fusion protein, no such transcripts are detected. Homozygotes are viable, fertile, and are comparable to wildtype littermates in size/weight. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).

Development

Portions of exons 7 and 8 were replaced with lacZ and neomycin resistance cassettes in (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant mice were backcrossed to 129S2/SvPas for at least 10 generations by the donating laboratory.

Allele

Symbol: Lrp5^tm1Grw
Name: targeted mutation 1, Georges Rawadi
MGI accession ID: MGI:3612485
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Lrp5
Marker name: low density lipoprotein receptor-related protein 5
Chromosome: 19
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting construct was designed to replace the 3' part of exon 7 and 5' part of exon 8 with a lac-Z-neo cassette by homologous recombination. The vector was intended to create a Lrp5-lacZ fusion protein, however, no Lrp5-containing transcripts were detected by Northern blot, and no Lrp5 or Lrp5-lacZ fusion proteins were detected by Western blot or histochemical staining of liver and bone.