Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).
Matthew Warman, Boston Children's Hospital, Harvard Medical School
Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. Expression of the targeted gene is not detectable in Western blots of liver, kidney and bone. Although the targeting construct was intended to generate a lacZ fusion protein, no such transcripts are detected. Homozygotes are viable, fertile, and are comparable to wildtype littermates in size/weight. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).
Portions of exons 7 and 8 were replaced with lacZ and neomycin resistance cassettes in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were backcrossed to 129S2/SvPas for at least 10 generations by the donating laboratory.
|Allele Name||targeted mutation 1, Georges Rawadi|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Lrp5, low density lipoprotein receptor-related protein 5|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting construct was designed to replace the 3' part of exon 7 and 5' part of exon 8 with a lac-Z-neo cassette by homologous recombination. The vector was intended to create a Lrp5-lacZ fusion protein, however, no Lrp5-containing transcripts were detected by Northern blot, and no Lrp5 or Lrp5-lacZ fusion proteins were detected by Western blot or histochemical staining of liver and bone.|
|Mutations Made By|| |
Georges Rawadi, Prostrakan Pharmaceuticals
When maintained as a live colony, heterozygotes or homozygotes may be bred. Due to increased skeletal fragility heterozygous female x homozygous male crosses might be considered. Heterozygous crosses are reported to produce litters with expected Mendelian genotypic ratios.
When using the 129S.129-Lrp5tm1Grw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012668 in your Materials and Methods section.
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