Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).
Matthew Warman, Boston Children's Hospital, Harvard Medical School
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Lrp5 | low density lipoprotein receptor-related protein 5 |
Homozygous Lrp5 (low density lipoprotein receptor-related protein 5) targeted mutation mice show significantly reduced bone mineral density (BMD) because of a deficiency in osteoblast number and function. Expression of the targeted gene is not detectable in Western blots of liver, kidney and bone. Although the targeting construct was intended to generate a lacZ fusion protein, no such transcripts are detected. Homozygotes are viable, fertile, and are comparable to wildtype littermates in size/weight. This strain may be useful in studies of bone mass and act as a model for Osteoporosis-Pseudoglioma syndrome (OPPG).
Portions of exons 7 and 8 were replaced with lacZ and neomycin resistance cassettes in (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were backcrossed to 129S2/SvPas for at least 10 generations by the donating laboratory.
Allele Name | targeted mutation 1, Georges Rawadi |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Lrp5- |
Gene Symbol and Name | Lrp5, low density lipoprotein receptor-related protein 5 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 19 |
Molecular Note | A targeting construct was designed to replace the 3' part of exon 7 and 5' part of exon 8 with a lac-Z-neo cassette by homologous recombination. The vector was intended to create a Lrp5-lacZ fusion protein, however, no Lrp5-containing transcripts were detected by Northern blot, and no Lrp5 or Lrp5-lacZ fusion proteins were detected by Western blot or histochemical staining of liver and bone. |
Mutations Made By | Georges Rawadi, Prostrakan Pharmaceuticals |
When maintained as a live colony, heterozygotes or homozygotes may be bred. Due to increased skeletal fragility heterozygous female x homozygous male crosses might be considered. Heterozygous crosses are reported to produce litters with expected Mendelian genotypic ratios.
When using the 129S.129-Lrp5tm1Grw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012668 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Lrp5<tm1Grw> |
Frozen Mouse Embryo | 129S.129-Lrp5<tm1Grw>/J | $2595.00 |
Frozen Mouse Embryo | 129S.129-Lrp5<tm1Grw>/J | $2595.00 |
Frozen Mouse Embryo | 129S.129-Lrp5<tm1Grw>/J | $3373.50 |
Frozen Mouse Embryo | 129S.129-Lrp5<tm1Grw>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.