B6;129S6-Ier3^tm1Raku/J

Stock number: 012664
Product name: gly96/IEX-1-
Research areas: Cardiovascular Research, Developmental Biology Research

Description

In this strain, exons1-2 of the endogenous mouse immediate early response 3 (Ier3) gene is replaced with a neo cassette, abolishing gene function. These mice may be useful for studying hypertension, cardiac hypertrophy, and cardiovascular physiology and disease.

Detailed description

In this strain, exons 1-2 of the endogenous mouse immediate early response 3 (Ier3) gene is replaced with a neo cassette, abolishing gene function. Homozygotes are viable, fertile, and normal in size. These mice develop elevated blood pressure, cardiac hypertrophy, and increased heart rate. No differences were found in the immune cells of the spleen, subcutaneous and mesenteric lymph nodes, thymus, or bone marrow of the gly96/IEX-1^-/- mice compared to wild-type. These mice may be useful for studying hypertension, cardiac hypertrophy, and cardiovascular physiology and disease.

Development

A targeting vector was designed to replace exons 1-2 of the immediate early response 3 (Ier3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6J to generate a colony of gly96/IEX-1^-/- mice. These mutant mice were maintained on a mixed C57BL/6J;129S6 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Allele

Symbol: Ier3^tm1Raku
Name: targeted mutation 1, Rajiv Kumar
MGI accession ID: MGI:3773690
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ier3
Marker name: immediate early response 3
Chromosome: 17
Strain of origin: 129S6/SvEvTac
Molecular note: Insertion of a targeting vector replaced both exons and intron 1 with a neo resistance cassette. RT-PCR did not detect transcript in eight tissues tested.