In this strain, exons1-2 of the endogenous mouse immediate early response 3 (Ier3) gene is replaced with a neo cassette, abolishing gene function. These mice may be useful for studying hypertension, cardiac hypertrophy, and cardiovascular physiology and disease.
Rajiv Kumar, Mayo Clinic
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ier3 | immediate early response 3 |
In this strain, exons 1-2 of the endogenous mouse immediate early response 3 (Ier3) gene is replaced with a neo cassette, abolishing gene function. Homozygotes are viable, fertile, and normal in size. These mice develop elevated blood pressure, cardiac hypertrophy, and increased heart rate. No differences were found in the immune cells of the spleen, subcutaneous and mesenteric lymph nodes, thymus, or bone marrow of the gly96/IEX-1-/- mice compared to wild-type. These mice may be useful for studying hypertension, cardiac hypertrophy, and cardiovascular physiology and disease.
A targeting vector was designed to replace exons 1-2 of the immediate early response 3 (Ier3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric mice were bred to C57BL/6J to generate a colony of gly96/IEX-1-/- mice. These mutant mice were maintained on a mixed C57BL/6J;129S6 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Rajiv Kumar |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | gly96/IEX-1-; IEX-1 KO |
Gene Symbol and Name | Ier3, immediate early response 3 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 17 |
Molecular Note | Insertion of a targeting vector replaced both exons and intron 1 with a neo resistance cassette. RT-PCR did not detect transcript in eight tissues tested. |
Mutations Made By | Rajiv Kumar, Mayo Clinic |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129S6-Ier3tm1Raku/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012664 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Ier3<tm1Raku> |
Frozen Mouse Embryo | B6;129S6-Ier3<tm1Raku>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Ier3<tm1Raku>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Ier3<tm1Raku>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S6-Ier3<tm1Raku>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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