These IGF-Iflox mutant mice may be useful in generating conditional mutations for studies of metabolism and growth.
Shoshana Yakar, Mount Sinai School of Medicine
These mice possess loxP sites on either side of exon 4 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in the cre-expressing tissue(s).
For example, when crossed to a strain expressing Cre recombinase in early mouse embryos (see Stock No. 003724), this mutant mouse strain may be useful in studies of Igf and postnatal development.
A neomycin cassette and loxP site were inserted downstream of exon 4, another loxP site was inserted upstream of exon 4. The construct was electroporated into 129 derived embryonic stem (ES) cells. The mice were then backcrossed to FVB/N for 12 generations. Upon arrival at The Jackson Laboratory the mice were crossed once to FVB/NJ to establish the colony.
|Allele Name||targeted mutation 1, Derek LeRoith|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Igf1floxed; IGF-I loxP; IGF-Iflox; LID; LI-IGF-I-|
|Gene Symbol and Name||Igf1, insulin-like growth factor 1|
|Strain of Origin||129|
|General Note||ES cells = 129/Sv|
|Molecular Note||A neomycin cassette and loxP sites were inserted flanking exon 4. The introduction of these mutations had no effect on the normal function of the gene.|
|Mutations Made By|| |
Dr. Derek LeRoith, Mount Sinai School of Medicine
When maintaining a live colony, these mice can be bred as homozygotes.
When using the IGF-Iflox mouse strain in a publication, please cite the originating article(s) and include JAX stock #012663 in your Materials and Methods section.