This transgenic mutant mouse strain may be useful in studies of growth and metabolism.
Shoshana Yakar, Mount Sinai School of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
These "HIT" transgenic mice overexpress human IGF1 specifically in the liver, with normal tissue IGF-I but have increased serum IGF-I levels. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that there is no difference between the phenotype exhibited by homozygotes compared to hemizygotes. Of note, the original publication indicates that the transgene contains rat insulin-like growth factor 1, Igf1, under the control of the rat Ttr, transthyretin, promoter, however, sequencing done by JAX indicates that human IGF1 driven by the mouse Ttr promoter.
A transgenic construct containing the human insulin-like growth factor 1, IGF1, under the control of the mouse Ttr, transthyretin, promoter was injected into FVB/N fertilized eggs. Of note, the original publication indicates that the transgene contains rat insulin-like growth factor 1, Igf1, under the control of the rat Ttr, transthyretin, promoter, however, sequencing done by JAX indicates that human IGF1 driven by the mouse Ttr promoter.
Founder line 1 was subsequently established. The mice were then backcrossed to FVB/N for 6 generations. Upon arrival at The Jackson Laboratory the mice were crossed with FVB/NJ (Stock No. 001800) once to establish the colony.
Expressed Gene | IGF1, insulin like growth factor 1, human |
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Site of Expression |
Allele Name | transgene insertion 1, Shoshana Yakar |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | HIT |
Gene Symbol and Name | Tg(Ttr-IGF1)1Sykr, transgene insertion 1, Shoshana Yakar |
Gene Synonym(s) | |
Promoter | Ttr, transthyretin, mouse, laboratory |
Expressed Gene | IGF1, insulin like growth factor 1, human |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | Originally submitted as rat sequence. However, susequent sequencing revealed the promoter to be mouse and the expressed sequence as human. |
Molecular Note | The transgene contains the human insulin-like growth factor 1 (IGF1) under the control of the mouse Ttr (transthyretin) promoter. Line 1 was created. |
When maintaining a live colony, these mice can be bred as hemizygotes or homozygotes.
When using the FVB-Tg(Ttr-IGF1)1Sykr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012662 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Ttr-Igf1)1Sykr |
Frozen Mouse Embryo | FVB-Tg(Ttr-IGF1)1Sykr/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Ttr-IGF1)1Sykr/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(Ttr-IGF1)1Sykr/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(Ttr-IGF1)1Sykr/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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