FVB-Tg(Ttr-IGF1)1Sykr/J

Stock number: 012662
Research areas: Cell Biology Research, Metabolism Research, Research Tools

Description

This transgenic mutant mouse strain may be useful in studies of growth and metabolism.

Detailed description

These "HIT" transgenic mice overexpress human IGF1 specifically in the liver, with normal tissue IGF-I but have increased serum IGF-I levels. Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that there is no difference between the phenotype exhibited by homozygotes compared to hemizygotes. Of note, the original publication indicates that the transgene contains rat insulin-like growth factor 1, Igf1, under the control of the rat Ttr, transthyretin, promoter, however, sequencing done by JAX indicates that human IGF1 driven by the mouse Ttr promoter.

Development

A transgenic construct containing the human insulin-like growth factor 1, IGF1, under the control of the mouse Ttr, transthyretin, promoter was injected into FVB/N fertilized eggs. Of note, the original publication indicates that the transgene contains rat insulin-like growth factor 1, Igf1, under the control of the rat Ttr, transthyretin, promoter, however, sequencing done by JAX indicates that human IGF1 driven by the mouse Ttr promoter. Founder line 1 was subsequently established. The mice were then backcrossed to FVB/N for 6 generations. Upon arrival at The Jackson Laboratory the mice were crossed with FVB/NJ (Stock No. 001800) once to establish the colony.

Allele

Symbol: Tg(Ttr-IGF1)1Sykr
Name: transgene insertion 1, Shoshana Yakar
MGI accession ID: MGI:4455320
Generation method: Transgenic
Attributes: Inserted expressed sequence
Synonyms: HIT
Marker symbol: Tg(Ttr-IGF1)1Sykr
Marker name: transgene insertion 1, Shoshana Yakar
Marker synonyms: HIT
Chromosome: UN
Strain of origin: FVB/N
Expressed genes: IGF1,insulin like growth factor 1,human
Molecular note: The transgene contains the human insulin-like growth factor 1 (IGF1) under the control of the mouse Ttr (transthyretin) promoter. Line 1 was created.
General note: Originally submitted as rat sequence. However, susequent sequencing revealed the promoter to be mouse and the expressed sequence as human.