B6.Cg-Tg(Ly6a-EGFP)G5Dzk/J

Stock number: 012643
Product name: Ly6a-GFP
Research areas: Research Tools

Description

Ly6a-GFP transgenic mice have an enhanced green fluorescent protein (EGFP) under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. These mice may be useful for the visualizing and sorting of hematopoietic stem cells.

Detailed description

Ly6a-GFP transgenic mice have an enhanced green fluorescent protein (EGFP) under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. Hemizygous Ly6a-GFP mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Ly6a promoter directs the expression of GFP in all functional repopulating adult hematopoietic stem cells (HSCs) in the adult bone marrow, and several other hematopoietic cell types [Ma et al. 2002 Stem Cells. (PMID:12456959)]. The GFP transgene expression pattern generally corresponds to that of Sca-1, a glycoprotein-I-linked cell-surface glycoprotein used routinely as a marker of adult HSCs. During embryonic development, the GFP transgene is expressed in all de novo-generated HSCs in the mouse embryonic aorta (only half of which are Sca-1 positive) and in all fetal liver HSCs [De Bruijn et al. 2002 Immunity. (PMID:12049719)]. Furthermore, the transgene is a potent marker of aortic hemogenic endothelium from which the first HSCs are generated [De Bruijn et al. 2002 Immunity. (PMID:12049719)], [Boisset et al. 2010 Nature. (PMID:20154729)], and [Kartalaei et al. 2014 JEM. (PMID:25547674)]. These mice may be useful for the visualizing and sorting of hematopoietic stem cells.

Development

The Ly6a-GFP transgene, created by Dr. Elaine Dzierzak (while at Erasmus MC), contains an enhanced green fluorescent protein (EGFP) and the simian virus 40 (SV40) polyadenylation (polyA) sequence under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. The construct was microinjected into fertilized (C57BL/10 x CBA)F1 oocytes, and offspring from founder line G5 were bred to (C57BL/10 x CBA)F1 mice to establish a colony. The donating investigator reported that these mice were then backcrossed to C57BL/6J (see SNP note below) for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J;C57BL/6N genetic background.

 

Allele

Symbol: Tg(Ly6a-EGFP)G5Dzk
Name: transgene insertion G5, Elaine Dzierzak
MGI accession ID: MGI:4437870
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Ly6a-EGFP)G5Dzk
Marker name: transgene insertion G5, Elaine Dzierzak
Chromosome: UN
Strain of origin: (C57BL/10 x CBA)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: GFP is expressed in all functional repopulating adult hematopoietic stem cells (HSCs) in the adult bone marrow, and several other hematopoietic cell types.
Molecular note: The Ly6a-GFP transgene contains an enhanced green fluorescent protein (EGFP) and the simian virus 40 (SV40) polyadenylation (polyA) sequence under the control of murine lymphocyte antigen 6 complex, locus A (Ly6a) promoter. Several lines were created. Line G5 has 8 copies of the transgene.