These HDAC2KO mutant mice lack exons 5-6 of the mouse histone deacetylase 2 (Hdac2) gene. This strain may be useful for studying the functional and structural synaptic changes and neuronal adaptive responses implicated in memory formation and storage.
Li-Huei Tsai, Massachusetts Institue of Technology
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Hdac2 | histone deacetylase 2 |
These HDAC2KO mutant mice lack exons 5-6 of the mouse histone deacetylase 2 (Hdac2) gene. Mice that are homozygous for this allele are viable, with reduced fertility, and are born at a twofold lower frequency than expected from a normal Mendelian ratio. Homozygous mutants are about 25% smaller than wild-type and heterozygote littermates. These mice have an increased synapse number and enhanced memory formation. This strain may be useful for studying the functional and structural synaptic changes and neuronal adaptive responses implicated in memory formation and storage.
A targeting vector was designed to insert a loxP site upstream of exon 5 and a loxP site downstream of exon 6 of the mouse histone deacetylase 2 (Hdac2) gene. The construct was electroporated into embryonic stem (ES) cells, and correctly targeted ES cells were injected into blastocysts. The resulting chimeric mice were bred to FVB/N to generate a colony of Hdac2L mice. These floxed mice were then bred to mice carrying cre recombinase on a C57BL/6 background to remove the floxed exons. These HDAC2KO mice were maintained on an FVB X C57BL/6 background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Allele Name | targeted mutation 1.2, Ronald DePinho |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | HDAC2KO |
Gene Symbol and Name | Hdac2, histone deacetylase 2 |
Gene Synonym(s) | |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 10 |
Molecular Note | LoxP sites were inserted upstream of exon 5 and downstream of exon 6. Mice carrying this allele were crossed with mice expressing cre recombinase in the germline. Gene inactivation was confirmed by a lack of signal in immunoblot analysis of homozygote brain extracts. The same analysis showed that expression of related Hdac1 protein was upregulated. |
Mutations Made By | Dr. Ronald DePinho, MD Anderson Cancer Center |
When maintaining a live colony, homozygous mice may be bred together. The donating investigator that heterozygotes crosses produce homozygous mutant mice at a twofold lower frequency than expected from a normal Mendelian ratio. While the homozygous mutant mice produced are viable and are capable of producing offspring, their fertility is reduced. Homozygous mutants are about 25% smaller than wild-type and heterozygote littermates.
When using the STOCK Hdac2tm1.2Rdp/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012640 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Hdac2<tm1.2Rdp> |
Frozen Mouse Embryo | STOCK Hdac2<tm1.2Rdp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Hdac2<tm1.2Rdp>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Hdac2<tm1.2Rdp>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Hdac2<tm1.2Rdp>/J Frozen Embryo | $3373.50 |
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