These mutant mice possess loxP sites flanking an HPRT minigene in intron 1 of the Cyp1a1 (cytochrome P450)gene and may be useful in organ specific studies involving aryl hydrocarbon (AHR) ligands, immunosuppression and metabolism of polycyclic aromatic hydrocarbon (PAH).
Daniel W Nebert, University of Cincinnati Medical Center
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in intestines and treatment with benzopyrene (BaP) results in immunosuppression and shortened lifespan. This mutant strain is useful in organ specific studies involving aryl hydrocarbon (AHR) ligands, immunosuppression and metabolism of polycyclic aromatic hydrocarbon (PAH).
A targeting vector containing a loxP-flanked HPRT minigene was inserted into intron 1 and a third loxP site was inserted into the 3' untranslated region. The construct was electroporated into 129P2/OlaHsd-dervied E14TG2a embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for at least 8 generations.
|Allele Name||targeted mutation 1, Daniel W Nebert|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Cyp1a1, cytochrome P450, family 1, subfamily a, polypeptide 1|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A HPRT minigene, flanked by loxP sites, was inserted into intron 1. An additional loxP site was inserted in the 3' untranslated region. Mice heterozygous for this allele were used to generate Cyp1a1tm1.1Dwn.|
|Mutations Made By|| |
Daniel Nebert, University of Cincinnati Medical Center
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6.129P2-Cyp1a1tm1Dwn/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32052 in your Materials and Methods section.