B6.129-Gria1^tm5Rlh/J

Stock number: 012612
Research areas: Neurobiology Research

Description

The serine 831 phosphorylation site was mutated to alanine in this Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1)) targeted mutation strain. Mutant protein is expressed in the brain. Appetitive incentive learning is defective in these mice, but performance of a number of other measures of motivated performance are unaffected. These mice may be useful in studies of normal and maladaptive forms of motivated behavior (e.g. addiction and eating disorders).

Detailed description

The serine 831 phosphorylation site was mutated to alanine in this Gria1 (glutamate receptor, ionotropic, AMPA1 (alpha 1)) targeted mutation strain. Normal GRIA1 protein expression is replaced by the mutant form in the brain. Normal basal synaptic transmission and plasticity are seen in young (3 week old) and adult (more than 3 month old) mice. Appetitive incentive learning is defective in these mice, but performance of a number of other measures of motivated performance are unaffected. These mice may be useful in studies of normal and maladaptive forms of motivated behavior (e.g. addiction and eating disorders).

Development

A Ser831Ala (TCC -> GCC) mutation was introduced to exon 17 of the targeted gene using (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Heterozygous mice were bred to CMV (cytomegaolovirus)-promoter cre mice to excise a floxed neomycin resistance cassette used as a selection marker. Cre was bred out in the next generation and this strain was backcrossed thirteen times to C57BL/6 by the donating laboratory.

Allele

Symbol: Gria1^tm5Rlh
Name: targeted mutation 5, Richard L Huganir
MGI accession ID: MGI:3793349
Generation method: Targeted
Attributes: Not Applicable
Marker symbol: Gria1
Marker name: glutamate receptor, ionotropic, AMPA1 (alpha 1)
Chromosome: 11
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: Exon 17 was replaced with one in which a nucleotide substitution resulted in an amio acid substitution of alanine for serine at position 831 (S831A). A neo cassette used as a selectable marker was removed by germline, cre-mediated recombination.